含碱性螺旋环螺旋域蛋白B8(BHLHB8)重组蛋白

Recombinant Basic Helix Loop Helix Domain Containing Protein B8 (BHLHB8)

MIST1; bHLHa15; BHLHB15; Class A Basic H

[ PROPERTIES ]

Source: Prokaryotic expression

Host: E.coli

Residues: Arg85~Thr189

Tags: N-terminal His Tag

Subcellular Location: Nucleus

Purity: > 95%

Traits: Freeze-dried powder

Buffer formulation: 20mM Tris, 150mM NaCl, pH8.0, containing 0.01% SKL, 5% Trehalose.

Original Concentration: 200μg/mL

Applications: Positive Control; Immunogen; SDS-PAGE; WB.

(May be suitable for use in other assays to be determined by the end user.)

Predicted isoelectric point: 10.8

Predicted Molecular Mass: 15.5kDa

Accurate Molecular Mass: 16kDa as determined by SDS-PAGE reducing conditions.

[ USAGE ]

Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

[ STORAGE AND STABILITY ]

Storage: Avoid repeated freeze/thaw cycles.

Store at 2-8°C for one month.

Aliquot and store at -80°C for 12 months.

Stability Test: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

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[ IDENTIFICATION ]

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