凋亡诱导因子(AIF)重组蛋白(Human,人) 

Recombinant Apoptosis Inducing Factor (AIF)

PDCD8; Programmed Cell Death protein 8; Apoptosis-inducing factor 1, mitochondrial

[ PROPERTIES ]

Host: E.coli

Residues: Gly103~Lys322

Tags: Two N-terminal Tags, His-tag and T7-tag

Accession: O95831

Subcellular Location: Mitochondrionintermembrane space. Mitochondrion innermembrane. Nucleus. Cytoplasm, perinuclearregion.

Purity: >95% 

Endotoxin Level: <1.0EU per 1μg(determined by the LAL method).

Formulation: Supplied as lyophilized form in 20mM Tris,150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT,0.01% sarcosyl, 5% trehalose, and preservative.

Predicted isoelectric point: 9.5

Predicted Molecular Mass:  Mass: 2

Applications: SDS-PAGE; WB; ELISA; IP.(May be suitable for use in other assays to be determined by the end user.)

[ USAGE ]

Reconstitute in sterile ddH?O.

[ STORAGE AND STABILITY ]

Storage:Avoid repeated freeze/thaw cycles.

Store at 2-8°C for one month.

Aliquot and store at -80°C for 12 months.

Stability Test: The thermal stability is described by the loss rate of thetargetprotein. The loss rate was determined by accelerated thermal degradationtest,that is, incubate the protein at 37°C for 48h, and no obvious degradationandprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this proteinislessthan 5% within the expiration date under appropriate storage condition.

[ SEQUENCES ]

The sequence of the target protein is listed below.

GLTPEQKQ KKAALSASEG EEVPQDKAPS HVPFLLIGGG TAAFAAARSI RARDPGARVLIVSEDPELPY MRPPLSKELW FSDDPNVTKT LRFKQWNGKE RSIYFQPPSF YVSAQDLPHIENGGVAVLTG KKVVQLDVRD NMVKLNDGSQ ITYEKCLIAT GGTPRSLSAI DRAGAEVKSRTTLFRKIGDF RSLEKISREV KSITIIGGGF LGSELACALG RK

[ REFERENCES ]

1. Susin S.A., et al. (1999) Nature 397:441-446.

2. Delettre C., et al. (2006) J. Biol. Chem. 281:6413-6427.

3. Delettre C., et al. (2006) J. Biol. Chem. 281:18507-18518.

4. Otera H., et al. (2005) EMBO J. 24:1375-1386.

[ IDENTIFICATION ]

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