甘露糖结合蛋白(MBL)重组蛋白(Human,人2) 

Recombinant Mannose Binding Lectin (MBL)

MBL2; COLEC1; HSMBPC; MBP1; MBP; Collectin-1; Mannose-binding protein C; Mannan Binding Protein; Mannose-Binding Lectin(Protein C)2,Soluble(Opsonic Defect)

[ PROPERTIES ]

Host: E.coli

Residues: Leu130~Ile248 (Accession # P11226),with two N-terminal Tags, His-tag and GST-tag.

Subcellular Location: Secreted

Purity: >95%

Endotoxin Level: <1.0EU per 1μg(determined by the LAL method).

formulation: Supplied as lyophilized form in PBS,pH7.4, containing 1mM DTT, 5% trehalose, 0.05%sarcosyl and preservative.

Predicted isoelectric point: 5.7

Predicted Molecular Mass: 45.0kDa

Applications: SDS-PAGE; WB; ELISA; IP.(May be suitable for use in other assays to be determined by the end user.)

[ USAGE ]

Reconstitute in sterile PBS, pH7.2-pH7.4.

[ STORAGE AND STABILITY ]

Storage:Avoid repeated freeze/thaw cycles.

Store at 2-8°C for one month.

Aliquot and store at -80°C for 12 months.

Stability Test: The thermal stability is described by the loss rate of thetargetprotein. The loss rate was determined by accelerated thermal degradationtest,that is, incubate the protein at 37°C for 48h, and no obvious degradationandprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this proteinislessthan 5% within the expiration date under appropriate storage condition.

[ SEQUENCES ]

The target protein is fused with two N-terminal Tags, His-tag and GST-tag, itssequence is listed below.MSPILGYWKI KGLVQPTRLL LEYLEEKYEE HLYERDEGDK WRNKKFELGL EFPNLPYYIDGDVKLTQSMA IIRYIADKHN MLGGCPKERA EISMLEGAVL DIRYGVSRIA YSKDFETLKVDFLSKLPEML KMFEDRLCHK TYLNGDHVTH PDFMLYDALD VVLYMDPMCL DAFPKLVCFKKRIEAIPQID KYLKSSKYIA WPLQGWQATF GGGDHPPKSD GSTSGSGHHH HHHSAGLVPRGSTAIGMKET AAAKFERQHM DSPDLGTLEV LFQGPLGSEF-L GKQVGNKFFL TNGEIMTFEKVKALCVKFQA SVATPRNAAE NGAIQNLIKE EAFLGITDEK TEGQFVDLTGNRLTYTNWNEGEPNNAGSDE DCVLLLKNGQ WNDVPCSTSH LAVCEFPI

[ REFERENCES ]

1. Ezekowitz R.A.B., et al. (1988) J. Exp. Med. 167:1034-1046.

2. Sastry K., et al. (1989) J. Exp. Med. 170:1175-1189.

3. Taylor M.E., et al. (1989) Biochem. J. 262:763-771.

4. Madsen H.O., et al. (1998) J. Immunol. 161:3169-3175.

[ IDENTIFICATION ]

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