间隙连接蛋白40(CX40)重组蛋白(Rat,大鼠) 

Recombinant Connexin 40 (CX40)

GJA5; GJ-A5; Gap Junction Protein Alpha 5

[ PROPERTIES ]

Source: Prokaryotic expression.

Host: E.coli

Residues: Ala50~Glu303

Tags: N-terminal His-Tag

Tissue Specificity: Lung.

Subcellular Location: Cell membrane; Multi-pass membrane protein. Celljunction, gap junction.

Purity: >98% 

Traits: Freeze-dried powder

Buffer formulation: 20mM Tris, 150mM NaCl, pH8.0, containing 1mMEDTA, 1mM DTT, 0.01% sarcosyl, 5%Trehalose and Proclin300.

Original Concentration: 200ug/mL

Applications: SDS-PAGE; WB; ELISA; IP; CoIP; Purification; Amine ReactiveLabeling.

(May be suitable for use in other assays to be determined by the end user.)

Predicted isoelectric point: 6.9

Predicted Molecular Mass: 30.1kDa

Accurate Molecular Mass: 20&16kDa as determined by SDS-PAGEreducingconditions.

Phenomenon explanation: 

The possible reasons that the actual band size differs fromthe predictedareasfollows:

1.Splice variants: Alternative splicing may create different sized proteins fromthe samegene.

2. Relative charge: The composition of amino acids may affects the charge of the protein.

3. Post-translational modification: Phosphorylation, glycosylation, methylation etc.

4. Post-translation cleavage: Many proteins are synthesized as pro-proteins, and then cleavedtogive the active form.

5. Polymerization of the target protein: Dimerization, multimerization etc.

[ USAGE ]

Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0mg/mL. Do not vortex.

[ STORAGE AND STABILITY ]

Storage:Avoid repeated freeze/thaw cycles.

Store at 2-8°C for one month.

Aliquot and store at -80°C for 12 months.

Stability Test: The thermal stability is described by the loss rate. Thelossratewas determined by accelerated thermal degradation test, that is, incubatetheprotein at 37°C for 48h, and no obvious degradation and precipitationwereobserved.The loss rate is less than 5% within the expiration dateunderappropriate storage condition.

[ SEQUENCE ]

[ IDENTIFICATION ]

特别提示:本公司的所有产品仅可用于科研实验,严禁用于临床医疗及其他非科研用途!以实际收货产品说明书为准,网站说明书仅供参考。