凋亡相关因子(FAS)重组蛋白(Cattle,牛) 

Recombinant Factor Related Apoptosis (FAS)

CD95; ALPS1A; ALPS1-A; APO1; APT1; FAS1; FASTM; TNFRSF6; Fas Receptor; TNF Receptor Superfamily Member 6; Tumor Necrosis Factor Receptor Superfamily Member 6

[ PROPERTIES ]

Host: E.coli

Residues: Leu37~Tyr274

Tags: Two N-terminal Tags, His-tag and T7-tag

Subcellular Location: Membrane; Single-pass type I membrane protein.

Purity: >95% 

Endotoxin Level: <1.0EU per 1μg (determined by the LAL method).

formulation: Supplied as lyophilized form in PBS, pH7.4, containing 5% trehalose, 0.01% sarcosyl.

Applications: SDS-PAGE; WB; ELISA; IP.

(May be suitable for use in other assays to be determined by the end user.)

Predicted isoelectric point: 6.5

Predicted Molecular Mass: 30.9kDa

Accurate Molecular Mass: 27kDa as determined by SDS-PAGE reducing conditions.

Note: The possible reasons that the actual band size differs from the predicted are as follows:

1.Splice variants: Alternative splicing may create different sized proteins from the same gene.

2. Relative charge: The composition of amino acids may affects the charge of the protein.

3. Post-translational modification: Phosphorylation, glycosylation, methylation etc.

4. Post-translation cleavage: Many proteins are synthesized as pro-proteins, and then cleavedto give the active form.

5. Polymerization of the target protein: Dimerization, multimerization etc.

[ USAGE ]

Reconstitute in sterile PBS, pH7.2-pH7.4

[ STORAGE AND STABILITY ]

Storage:Avoid repeated freeze/thaw cycles.

Store at 2-8°C for one month.

Aliquot and store at -80°C for 12 months.

Stability Test:  The thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal  egradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. (Referring from China Biological Products Standard, which was calculated by the Arrhenius equation.) The loss of this protein is less than 5% within the expiration date under appropriate storage condition.

[ SEQUENCES ]

The sequence of the target protein is listed below.

LNIT EANSCQEGLY REHQFCCQPC PPGKRKNGDC KRDGDTPECV LCSEGNEYTD

KSHHSDKCIR CSICDEEHGL EVEQNCTRTR NTKCRCKSNF FCNSSPCEHC NPCTTCEHGI

IEKCTPTSNT KCKGSRSHAN SLWALLILLI PIVLIIYKVV KSRERNKKND YCNSAASNDE

GRQLNLTDVD LGKYIPSIAE QMRITEVKEF VRKNGMEEAK IDDIMHDNVH ETAEQKVQLL

RNWY

[ IDENTIFICATION ]

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