CD34分子(CD34)重组蛋白 (Mouse,小鼠) 

Recombinant Cluster Of Differentiation 34 (CD34)

Hematopoietic progenitor cell antigen CD34

[ PROPERTIES ]

Host: E.coli

Residues: Thr36~Thr287,with two N-terminal Tags, His-tag and GST-tag.

Subcellular Location: Membrane; Single-passtype I membrane protein

Purity: >95% 

Endotoxin Level: <1.0EU per 1μg(determined by the LAL method).

Formulation: Supplied as lyophilized form in PBS,pH7.4, containing 5% sucrose, 0.01% sarcosyl.

Predicted isoelectric point: 5.4

Predicted Molecular Mass: 59.1kDa

Applications: SDS-PAGE; WB; ELISA; IP.

(May be suitable for use in other assays to be determined by the end user.)

[ USAGE ]

Reconstitute in sterile PBS, pH7.2-pH7.4.

[ STORAGE AND STABILITY ]

Storage:Avoid repeated freeze/thaw cycles.

Store at 2-8°C for one month.

Aliquot and store at -80°C for 12 months.

Stability Test: The thermal stability is described by the loss rate of thetargetprotein. The loss rate was determined by accelerated thermal degradationtest,that is, incubate the protein at 37°C for 48h, and no obvious degradationandprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this proteinislessthan 5% within the expiration date under appropriate storage condition.

[ SEQUENCES ]

The target protein is fused with two N-terminal Tags, His-tag and GST-tag, itssequence is listed below.

MSPILGYWKI KGLVQPTRLL LEYLEEKYEE HLYERDEGDK WRNKKFELGL EFPNLPYYIDGDVKLTQSMA IIRYIADKHN MLGGCPKERA EISMLEGAVL DIRYGVSRIA YSKDFETLKVDFLSKLPEML KMFEDRLCHK TYLNGDHVTH PDFMLYDALD VVLYMDPMCL DAFPKLVCFKKRIEAIPQID KYLKSSKYIA WPLQGWQATF GGGDHPPKSD GSTSGSGHHH HHHSAGLVPRGSTAIGMKET AAAKFERQHM DSPDLGTLEV LFQGPLGSEF -TETST QGISPSVPTNESVEENITSS IPGSTSHYLI YQDSSKTTPA ISETMVNFTV TSGIPSGSGT PHTFSQPQTSPTGILPTTSD SISTSEMTWK SSLPSINVSD YSPNNSSFEM TSPTEPYAYT SSSAPSAIKGEIKCSGIREV RLAQGICLEL SEASSCEEFK KEKGEDLIQI LCEKEEAEAD AGASVCSLLLAQSEVRPECL LMVLANSTEL PSKLQLMEKH QSDLRKLGIQ SFNKQDIGSHQSYSRKT

[ REFERENCES ]

1. Brown J., et al. (1991) Int. Immunol. 3:175-184.

2. Suda J., et al. (1992) Blood 79:2288-2295.

3. Gundry R.L., et al. (2009) Mol. Cell. Proteomics 8:2555-2569.

4. Ishida A., et al. (2002) Exp. Hematol. 30:361-365.

[ IDENTIFICATION ]

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