内皮脂肪酶(LIPG)重组蛋白(Rat,大鼠) 

Recombinant Lipase, Endothelial (LIPG)

EL; EDL; Endothelial Lipase; Endothelial cell-derived lipase

[ PROPERTIES ]

Host: E.coli

Residues: Gly203~Lys471 ,with two N-terminal Tags, His-tag and T7-tag.

Subcellular Location: Secreted

Purity: >95% 

Endotoxin Level: <1.0EU per 1μg(determined by the LAL method).

Formulation: Supplied as lyophilized form in 20mM Tris,500mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT,0.01% sarcosyl, 5% trehalose, and preservative.

Applications: SDS-PAGE; WB; ELISA; IP.

(May be suitable for use in other assays to be determined by the end user.)

Predicted isoelectric point: 9.2

Predicted Molecular Mass: 34.6kDa

[ USAGE ]

Reconstitute in sterile PBS, pH7.2-pH7.4.

[ STORAGE AND STABILITY ]

Storage:Avoid repeated freeze/thaw cycles.

Store at 2-8°C for one month.

Aliquot and store at -80°C for 12 months.

Stability Test: The thermal stability is described by the loss rate of thetargetprotein. The loss rate was determined by accelerated thermal degradationtest,that is, incubate the protein at 37°C for 48h, and no obvious degradationandprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this proteinislessthan 5% within the expiration date under appropriate storage condition.

[ SEQUENCES ]

The target protein is fused with two N-terminal Tags, His-tag and T7-tag, itssequence is listed below.

MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGS- GVDINRRL SPDDADFVDVLHTYTLSFGL SIGIRMPVGH IDIYPNGGDF QPGCGFNDVM GSFAYGTISE MVKCEHERAVHLFVDSLVNQ DKPSFAFQCT DPNRFKRGIC LSCRKNRCNN IGYNAKKMRK KRNSKMYLKTRAGMPFRVYH YQLKVHMFSY KNSGDIQPDL YITLYGSNAD SQNLPLEIVE KIELNATNTFLVYTEEYLGD LFKIRLTWEG VSSSWYNLWN EFRSYLSQPS SPSRELHIRR IRVKSGETQRKVAFCVQDPM K

[ REFERENCES ]

1. Shimokawa Y., et al. (2005) Cardiovasc. Res. 66:594-600.

2. Bonne A.C.M., et al. (2001) DNA Seq. 12:285-287.

3. Stevenson F.T., et al. (2001) Kidney Int. 59:2062-2068.

4. Hutter C.M., et al. (2006) Atherosclerosis 185:78-86.

[ IDENTIFICATION ]

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