CD14分子(CD14)重组蛋白 (Rat,大鼠) 

Recombinant Cluster Of Differentiation 14 (CD14)

Monocyte differentiation antigen CD14; Myeloid cell-specific leucine-rich glycoprotein

[ PROPERTIES ]

Host: E.coli

Residues: Ser18~Val372 , with two N-terminal Tags, His-tag and GST-tag.

Subcellular Location: Cell membrane; Lipid-anchor, GPI-anchor

Purity: >95% 

Endotoxin Level: <1.0EU per 1μg (determined by the LAL method).

Formulation: Supplied as lyophilized form in PBS, pH7.4, containing 5% sucrose, 0.01% sarcosyl.

Applications: SDS-PAGE; WB; ELISA; IP.

(May be suitable for use in other assays to be determined by the end user.)

Predicted isoelectric point: 5.7

Predicted Molecular Mass: 69.9kDa

[ USAGE ]

Reconstitute in sterile PBS, pH7.2-pH7.4.

[ STORAGE AND STABILITY ]

Storage:Avoid repeated freeze/thaw cycles.

Store at 2-8°C for one month.

Aliquot and store at -80°C for 12 months.

Stability Test: The thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37oC for 48h, and no obvious degradation and precipitation were observed. (Referring from China Biological Products Standard, which was calculated by the Arrhenius equation.) The loss of this protein is less than 5% within the expiration date under appropriate storage condition.

[ SEQUENCES ]

The target protein is fused with two N-terminal Tags, His-tag and GST-tag, its sequence is listed below.

MSPILGYWKI KGLVQPTRLL LEYLEEKYEE HLYERDEGDK WRNKKFELGL EFPNLPYYID

GDVKLTQSMA IIRYIADKHN MLGGCPKERA EISMLEGAVL DIRYGVSRIA YSKDFETLKV

DFLSKLPEML KMFEDRLCHK TYLNGDHVTH PDFMLYDALD VVLYMDPMCL DAFPKLVCFK

KRIEAIPQID KYLKSSKYIA WPLQGWQATF GGGDHPPKSD GSTSGSGHHH HHHSAGLVPR

GSTAIGMKET AA AKFE RQ HM DSP D LG T LEV LFQ GP LGS -SPA TPE P CE L DQ D

EESVRCYCNF SDPQPNWSSA FLCAGAEDVE FYGGGRSLEY LLKRVDTEAN LGQYTDIIRS

LPLKRLTVRS ARVPTQILFG TLRVLGYSGL RELTLENLEV TGTALSPLLD ATGPDLNTLS

LRNVSWATTD TWLAELQQWL KPGLKVLSIA QAHSLNFSCK QVGVFPALAT LDLSDNPELG

EKGLISALCP HKFPTLQVLA LRNAGMETTS GVCSALAAAR VPLQALDLSH NSLRDTAGTP

SCDWPSQLNS LNLSFTGLEH VPKGLPAKLS VLDLSYNRLD RKPRPEELPE VGSLSLTGNP

FLHSESQSEA YNSGVVIATA LSPGSAGLSG TLALLLGHRL FV

[ IDENTIFICATION ]

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