低氧诱导因子1α(HIF1a)重组蛋白 (Gallus,鸡2) 

Recombinant Hypoxia Inducible Factor 1 Alpha (HIF1a)

HIF1-A; MOP1; PASD8; Basic Helix-Loop-Helix Transcription Factor; ARNT-interacting protein; Basic-helix-loop-helix-PAS protein MOP1; Class E basic helix-loop-helix protein 78

[ PROPERTIES ]

Host: E.coli

Residues: Asp238~Lys389 ,with N-terminal His-Tag

Subcellular Location: Cytoplasm. Nucleus.

Purity: >95% 

Endotoxin Level: <1.0EU per 1μg(determined by the LAL method).

Formulation: Supplied as lyophilized form in 20mM Tris,500mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT,0.01% sarcosyl, 5% trehalose, and preservative.

Applications: SDS-PAGE; WB; ELISA; IP.

(May be suitable for use in other assays to be determined by the end user.)

Predicted isoelectric point: 9.3

Predicted Molecular Mass: 18.9kDa

[ USAGE ]

Reconstitute in ddH?O

[ STORAGE AND STABILITY ]

Storage:Avoid repeated freeze/thaw cycles.

Store at 2-8°C for one month.

Aliquot and store at -80°C for 12 months.

Stability Test: The thermal stability is described by the loss rate of thetargetprotein. The loss rate was determined by accelerated thermal degradationtest,that is, incubate the protein at 37°C for 48h, and no obvious degradationandprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this proteinislessthan 5% within the expiration date under appropriate storage condition.

[ SEQUENCES ]

The target protein is fused with N-terminal His-Tag, its sequence is listedbelow.

MGHHHHHHSGS-DSK TFLSRHSLDM KFSYCDERIT ELMGYEPEEL LGRSIYEYYHALDSDHLTKT HHDMFTKGQV TTGQYRMLAK QGGYVWVETQ ATVIYNTKNS QPQCIVCVNYVLSGIVQKDL IFSLGQTECM LKPVESPEMK MTKIFSKDDW DDTNSLFEK

[ REFERENCES ]

1. Takahashi T. (1998) Submitted to the EMBL/GenBank/DDBJ databases.

2. Ratcliffe PJ. (2003) Blood Purif. 20 (5): 445–50.

3. van Uden P., et al. (2008) Biochem J. 412 (3): 477–484.

4. Cho, S; et al. (2001) FEBS Lett. (Netherlands) 498 (1): 62–6.

[ IDENTIFICATION ]

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