碱性鞘磷脂磷酸二酯酶(Alk-Smase)重组蛋白(Rat,大鼠) 

Recombinant Alkaline Sphingomyelinase (Alk-Smase)

ENPP7; E-NPP 7; NPP-7; Ectonucleotide Pyrophosphatase/Phosphodiesterase 7; Sphingomyelin Phosphodiesterase; Intestinal alkaline sphingomyelinase

[ PROPERTIES ]

Source: Prokaryotic expression

Host: E.coli

Residues: Gly22~Val439

Tags: N-terminal His Tag

Homology: Human 78%, Mouse 90%

Tissue Specificity: Gejunum

Subcellular Location: Membrane; Single-pass type I membrane protein.

Purity: >95% 

Endotoxin Level: <1.0EU per 1μg (determined by the LAL method).

Traits: Freeze-dried powder

Buffer formulation: 20mM Tris, 150mM NaCl, pH8.0, containing 1mMEDTA, 1mM DTT, 0.01% sarcosyl, 5%Trehalose and Proclin300.

Original Concentration: 200ug/mL

Applications: SDS-PAGE; WB; ELISA; IP; CoIP; Reporter Assays; Purification;Amine Reactive Labeling.

(May be suitable for use in other assays to be determined by the end user.)

Predicted isoelectric point: 6.6

Predicted Molecular Mass: 51.5kDa

Accurate Molecular Mass: 51kDa as determined by SDS-PAGE reducingconditions.

[ USAGE ]

Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0mg/mL. Do not vortex.

[ STORAGE AND STABILITY ]

Storage:Avoid repeated freeze/thaw cycles.

Store at 2-8°C for one month.

Aliquot and store at -80°C for 12 months.

Stability Test: The thermal stability is described by the loss rate. Thelossratewas determined by accelerated thermal degradation test, that is, incubatetheprotein at 37°C for 48h, and no obvious degradation and precipitationwereobserved.The loss rate is less than 5% within the expiration dateunderappropriate storage condition.

[ SEQUENCE ]

[ IDENTIFICATION ]

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