60kD热休克蛋白(Hsp60)重组蛋白 (Human,人2) 

Recombinant Heat Shock Protein 60 (Hsp60)

HSPD1; GROEL; CPN60; HuCHA60; Heat Shock 60kD Protein 1, Chaperonin; Spastic Paraplegia 13,Autosomal Dominant; 60 kDa chaperonin; Mitochondrial matrix protein P1; P60 lymphocyte protein

[ PROPERTIES ]

Host: E.coli

Residues: Ala172~Gly343 ,with N-terminal His-Tag.

Subcellular Location: Mitochondrion matrix.

Purity: >95% 

Endotoxin Level: <1.0EU per 1μg(determined by the LAL method).

Formulation: Supplied as lyophilized form in PBS,pH7.4, containing 5% trehalose, 0.01% sarcosyl.

Predicted isoelectric point: 6.2

Predicted Molecular Mass: 19.8kDa

Applications: SDS-PAGE; WB; ELISA; IP.

(May be suitable for use in other assays to be determined by the end user.)

[ USAGE ]

Reconstitute in sterile PBS, pH7.2-pH7.4.

[ STORAGE AND STABILITY ]

Storage:Avoid repeated freeze/thaw cycles.

Store at 2-8°C for one month.

Aliquot and store at -80°C for 12 months.

Stability Test: The thermal stability is described by the loss rate of thetargetprotein. The loss rate was determined by accelerated thermal degradationtest,that is, incubate the protein at 37°C for 48h, and no obvious degradationandprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this proteinislessthan 5% within the expiration date under appropriate storage condition.

[ SEQUENCES ]

The target protein is fused with N-terminal His-Tag, its sequence is listedbelow.MGHHHHHHSGS-ATISANGDK EIGNIISDAM KKVGRKGVIT VKDGKTLNDE LEIIEGMKFDRGYISPYFIN TSKGQKCEFQ DAYVLLSEKK ISSIQSIVPA LEIANAHRKP LVIIAEDVDGEALSTLVLNR LKVGLQVVAV KAPGFGDNRK NQLKDMAIAT GGAVFGEEGL TLNLEDVQPHDL

[ REFERENCES ]

1. Magen D., et al. (2008) Am. J. Hum. Genet. 83:30-42.

2. Merle N., et al. (2012) Mitochondrion 12:441-448.

3. Peng C., et al. (2011) Mol. Cell. Proteomics 10:M111.012658.01-M111.012658.12.

4. Burkard T.R., et al. (2011) BMC Syst. Biol. 5:17-17.

[ IDENTIFICATION ]

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