细胞色素P450家族成员7A1(CYP7A1)重组蛋白(Mouse,小鼠) 

Recombinant Cytochrome P450 7A1 (CYP7A1)

CYP7; CYP7-A1; CYPVII; Cholesterol 7 Alpha Hydroxylase; Cytochrome P450 Family 7 Subfamily A Polypeptide 1

[ PROPERTIES ]

Host: E.coli

Residues: Leu227~Leu467 ,with two N-terminal Tags, His-tag and T7-tag.

Subcellular Location: Endoplasmic reticulummembrane; Peripheral membrane protein.Microsome membrane.

Purity: >95% 

Endotoxin Level: <1.0EU per 1μg(determined by the LAL method).

Formulation: Supplied as lyophilized form in PBS,pH7.4, containing 5% sucrose, 0.01% sarcosyl.

Predicted isoelectric point: 6.9

Predicted Molecular Mass: 31.5kDa

Applications: SDS-PAGE; WB; ELISA; IP.

(May be suitable for use in other assays to be determined by the end user.)

[ USAGE ]

Reconstitute in sterile PBS, pH7.2-pH7.4.

[ STORAGE AND STABILITY ]

Storage:Avoid repeated freeze/thaw cycles.

Store at 2-8°C for one month.

Aliquot and store at -80°C for 12 months.

Stability Test: The thermal stability is described by the loss rate of thetargetprotein. The loss rate was determined by accelerated thermal degradationtest,that is, incubate the protein at 37°C for 48h, and no obvious degradationandprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this proteinislessthan 5% within the expiration date under appropriate storage condition.

[ SEQUENCES ]

The target protein is fused with two N-terminal Tags, His-tag and T7-tag, itssequence is listed below.

MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF - LFKT AHKARERLAESLKHKNLYMR DQVSELIRLR MFLNDTLSTF DDMEKAKTHL VILWASQANT IPATFWSLFQMIRSPEAMKA ASEEVNGALQ SAGQELSSGG NAIYLDQEQL NNLPVLDSII KEALRLSSASLNIRTAKEDF TLHLEDGSYN IRKDDIIALY PQLMHLDPEI YPDPLTFKYD RYLDESGKAKTTFYRNGNKL KYFYMPFGSG ATICPGRLFA VQEIKQFLIL MLSYFEL

[ IDENTIFICATION ]

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