嗜铬蛋白A(CHGA)重组蛋白 (Human,人) 

Recombinant Chromogranin A (CHGA)

CGA; SP-I; Parathyroid Secretory Protein

[ PROPERTIES ]

Source: Prokaryotic expression. 

Host: E. coli

Residues: Gln15~Gly148

Tags: N-terminal His-Tag

Tissue Specificity: Liver, Stomach, Brain, Pancreas. 

Subcellular Location: Cytoplasmic vesicle, secretory vesicle lumen..

Purity: >98%

Traits: Freeze-dried powder

Buffer formulation: 20mM Tris, 150mM NaCl, pH8.0, containing 1mMEDTA, 1mM DTT, 0.01% sarcosyl, 5%Trehalose and Proclin300. 

Original Concentration: 200ug/mL

Applications: SDS-PAGE; WB; ELISA; IP; CoIP; Reporter Assays; Purification;Amine Reactive Labeling. 

(May be suitable for use in other assays to be determined by the end user.)

Predicted isoelectric point: 5.1

Predicted Molecular Mass: 16.4kDa

Accurate Molecular Mass: 20kDa as determined by SDS-PAGE reducingconditions.

[ USAGE ]

Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0mg/mL. Do not vortex.

[ STORAGE AND STABILITY ]

Storage: Avoid repeated freeze/thaw cycles. 

Store at 2-8℃ for one month. 

Aliquot and store at -80℃ for 12 months. 

Stability Test: The thermal stability is described by the loss rate. Thelossratewas determined by accelerated thermal degradation test, that is, incubatetheprotein at 37℃ for 48h, and no obvious degradation and precipitationwereobserved.The loss rate is less than 5% within the expirationdateunderappropriate storage condition. 

[ SEQUENCE ]

[ IDENTIFICATION ]

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