圆柱瘤蛋白(CYLD)重组蛋白(Human,人) 

Recombinant Cylindromatosis (CYLD)

CDMT; CYLD1; CYLDI; EAC; USPL2; Turban Tumor Syndrome; Ubiquitin Carboxyl-Terminal Hydrolase CYLD; Ubiquitin Specific Peptidase Like 2; Deubiquitinating enzyme CYLD

[ PROPERTIES ]

Host: E.coli

Residues: Glu170~Gly338 , with two N-terminal Tags, His-tag and T7-tag.

Subcellular Location: Cytoplasm, perinuclear region. Cytoskeleton. Cell membrane; Peripheral membrane protein; Cytoplasmic side.

Purity: >95%

Endotoxin Level: <1.0EU per 1μg (determined by the LAL method).

Formulation: Supplied as lyophilized form in PBS, pH7.4, containing 5% trehalose, 0.01% sarcosyl.

Applications: SDS-PAGE; WB; ELISA; IP.

(May be suitable for use in other assays to be determined by the end user.)

Predicted isoelectric point: 4.9

Predicted Molecular Mass: 22.1kDa

[ USAGE ]

Reconstitute in sterile PBS, pH7.2-pH7.4.

[ STORAGE AND STABILITY ]

Storage:Avoid repeated freeze/thaw cycles.

Store at 2-8°C for one month.

Aliquot and store at -80°C for 12 months.

Stability Test: The thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal  egradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. (Referring from China Biological Products Standard, which was calculated by the Arrhenius equation.) The loss of this protein is less than 5% within the expiration date under appropriate storage condition.

[ SEQUENCES ]

The target protein is fused with two N-terminal Tags, His-tag and T7-tag, its sequence is listed below.

MGSS H H H H H H SS G LV P R GS H MASM T G G Q QM GRGS E F - E GR G Q G F T D GV

YQGKQLFQCD EDCGVFVALD KLELIEDDDT ALESDYAGPG DTMQVELPPL EINSRVSLKV

GETIESGTVI FCDVLPGKES LGYFVGVDMD NPIGNWDGRF DGVQLCSFAC VESTILLHIN

DIIPESVTQE RRPPKLAFMS RGVGDKGSSS HNKPKATG

[ IDENTIFICATION ]

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