脂肪酸转运蛋白5(SLC27A5)重组蛋白(Mouse,小鼠2)
Recombinant Solute Carrier Family 27 Member 5 (SLC27A5)
FATP5; ACSB; ACSVL6; FACVL3; VLACSR; BACS; VLCSH2; Fatty Acid Coenzyme A Ligase Very Long Chain 3; Fatty Acid Transport Protein 5; Bile Acyl CoA Synthetase
[ PROPERTIES ]
Host: E.coli
Residues: Glu471~Leu689,with two N-terminal Tags, His-tag and GST-tag.
Subcellular Location: Endoplasmic reticulummembrane; Multi-pass membrane protein.
Purity: >95%
Endotoxin Level: <1.0EU per 1μg(determined by the LAL method).
Formulation: Supplied as lyophilized form in PBS,pH7.4, containing 5% sucrose, 0.01% sarcosyl.
Applications: SDS-PAGE; WB; ELISA; IP.
(May be suitable for use in other assays to be determined by the end user.)
Predicted isoelectric point: 6.1
Predicted Molecular Mass: 56.6kDa
[ USAGE ]
Reconstitute in sterile PBS, pH7.2-pH7.4.
[ STORAGE AND STABILITY ]
Storage:Avoid repeated freeze/thaw cycles.
Store at 2-8°C for one month.
Aliquot and store at -80°C for 12 months.
Stability Test: The thermal stability is described by the loss rate of thetargetprotein. The loss rate was determined by accelerated thermal degradationtest,that is, incubate the protein at 37°C for 48h, and no obvious degradationandprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this proteinislessthan 5% within the expiration date under appropriate storage condition..
[ SEQUENCES ]
The target protein is fused with two N-terminal Tags, His-tag and GST-tag, itssequence is listed below.
MSPILGYWKI KGLVQPTRLL LEYLEEKYEE HLYERDEGDK WRNKKFELGL EFPNLPYYIDGDVKLTQSMA IIRYIADKHN MLGGCPKERA EISMLEGAVL DIRYGVSRIA YSKDFETLKVDFLSKLPEML KMFEDRLCHK TYLNGDHVTH PDFMLYDALD VVLYMDPMCL DAFPKLVCFKKRIEAIPQID KYLKSSKYIA WPLQGWQATF GGGDHPPKSD GSTSGSGHHH HHHSAGLVPRGSTAIGMKET AAAKFERQHM DSPDLGTLEV LFQGPLGSEF- ETAEPLRDKQGFCIPVEPGKPGLLLTKVRK NQPFLGYRGS QAESNRKLVA NVRRVGDLYF NTGDVLTLDQEGFFYFQDRLGD T F RWK GE N VS T GE V E CV L SS L D F L E E V N VY GV P V P G CE GK VG MA AV K LAPGKTFDGQK LYQHVRSWLP AYATPHFIRI QDSLEITNTY KLVKSRLVRE GFDVGIIADPLYILDNKAQT FRSLMPDVYQ AVCEGTWNL
[ IDENTIFICATION ]

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