磷酸烯醇式丙酮酸羧激酶2(PCK2)重组蛋白 (Human,人)  

Recombinant Phosphoenolpyruvate Carboxykinase 2, Mitochondrial (PCK2)

PEPCK; PEPCK2

[ PROPERTIES ]

Source: Prokaryotic expression

Host: E.coli

Residues: Leu33~Val198

Tags: N-terminal His Tag

Subcellular Location: Mitochondrion. 

Purity: >99%

Traits: Freeze-dried powder

Buffer formulation: 100mM NaHCO₃, 500mM NaCl, pH8.3, containing1mMEDTA, 1mM DTT, 0.01% sarcosyl, 5%Trehalose and Proclin300.

Original Concentration: 200μg/mL

Applications: SDS-PAGE; WB; ELISA; IP; CoIP; Purification; Amine ReactiveLabeling.

(May be suitable for use in other assays to be determined by the end user.)

Predicted isoelectric point: 7.9

Predicted Molecular Mass: 21.8kDa

Accurate Molecular Mass: 24kDa as determined by SDS-PAGE reducingconditions.

[ USAGE ]

Reconstitute in 100mM NaHCO₃, 500mM NaCl (pH8.3) to a concentrationof0.1-1.0 mg/mL. Do not vortex.

[ STORAGE AND STABILITY ]

Storage: Avoid repeated freeze/thaw cycles.

Store at 2-8℃ for one month.

Aliquot and store at -80℃ for 12 months.

Stability Test: The thermal stability is described by the loss rate. The loss rate was determinedby accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and noobvious degradation and precipitation were observed. The loss rate is less than 5% within theexpiration date under appropriate storage condition.

[ SEQUENCE ]

[ IDENTIFICATION ]

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