肌球蛋白轻链激酶3(MYLK3)重组蛋白(Mouse,小鼠) 

Recombinant Myosin Light Chain Kinase 3 (MYLK3)

MLCK3; MLCK; MLCK2; caMLCK; MLC Kinase; Cardiac Myosin Light Chain Kinase; Cardiac-MyBP-C-associated Ca/CaM kinase

[ PROPERTIES ]

Host: E.coli

Residues: Gly488~Pro750 ,with N-terminal His-Tag

Subcellular Location: Cytoplasm.

Purity: >95% 

Endotoxin Level: <1.0EU per 1μg(determined by the LAL method).

formulation: Supplied as lyophilized form in PBS,pH7.4, containing 5% sucrose, 0.01% sarcosyl.

Predicted isoelectric point: 6.0

Predicted Molecular Mass: 31.7kDa

Applications: SDS-PAGE; WB; ELISA; IP.

(May be suitable for use in other assays to be determined by the end user.)

[ USAGE ]

Reconstitute in sterile PBS, pH7.2-pH7.4.

[ STORAGE AND STABILITY ]

Storage:Avoid repeated freeze/thaw cycles.

Store at 2-8°C for one month.

Aliquot and store at -80°C for 12 months.

Stability Test: The thermal stability is described by the loss rate of thetargetprotein. The loss rate was determined by accelerated thermal degradationtest,that is, incubate the protein at 37°C for 48h, and no obvious degradationandprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this proteinislessthan 5% within the expiration date under appropriate storage condition.

[ SEQUENCES ]

The target protein is fused with N-terminal His-Tag, its sequence is listedbelow.

MGHHHHHHSG SEF-GYT VSQHEVLGGG RFGQVHRCTE RSTGLALAAK IIKVKNVKDREDVKNEVNIM NQLSHVNLIQ LYDAFESKSS FTLIMEYVDG GELFDRITDE KYHLTELDVVLFTRQICEGV HYLHQHYILH LDLKPENILC VSQTGHQIKI IDFGLARRYK PREKLKVNFGTPEFLAPEVV NYEFVSFPTD MWSVGVITYM LLSGLSPFLG ETDAETMNFI VNCSWDFDADTFKGLSEEAK DFVSRLLVKE KSCRMSATQC LKHEWLSHLP

[ IDENTIFICATION ]

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