骨髓基质细胞抗原1(BST1)重组蛋白 (Human,人) 

Recombinant Bone Marrow Stromal Cell Antigen 1 (BST1)

CD157; ADP-Ribosyl Cyclase 2; ADP-ribosyl cyclase/cyclic ADP-ribose hydrolase 2

[ PROPERTIES ]

Host: E.coli

Residues: Arg63~Ala313,with two N-terminal Tags, His-tag and T7-tag.

Subcellular Location: Secreted

Purity: >95%

Endotoxin Level: <1.0EU per 1μg(determined by the LAL method).

Formulation: Supplied as lyophilized form in PBS,pH7.4, containing 5% sucrose, 0.01% sarcosyl.

Applications: SDS-PAGE; WB; ELISA; IP.

(May be suitable for use in other assays to be determined by the end user.)

Predicted isoelectric point: 6.9

Predicted Molecular Mass: 32.3kDa

[ USAGE ]

Reconstitute in sterile PBS, pH7.2-pH7.4.

[ STORAGE AND STABILITY ]

Storage:Avoid repeated freeze/thaw cycles.

Store at 2-8°C for one month.

Aliquot and store at -80°C for 12 months.

Stability Test: The thermal stability is described by the loss rate of thetargetprotein. The loss rate was determined by accelerated thermal degradationtest,that is, incubate the protein at 37°C for 48h, and no obvious degradationandprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this proteinislessthan 5% within the expiration date under appropriate storage condition.

[ SEQUENCES ]

The target protein is fused with two N-terminal Tags, His-tag and T7-tag, itssequence is listed below.

MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF-RNKNCTAI WEAFKVALDKDPCSVLPSDY DLFINLSRHS IPRDKSLFWE NSHLLVNSFA DNTRRFMPLS DVLYGRVADFLSW C R QK N DS GL DYQS CPT S ED CE N NPV DS FWK RASIQY S KDS SGVI HVMLNGSEPTGAY PIKGFFADYE IPNLQKEKIT RIEIWVMHEI GGPNVESCGE GSMKVLEKRLKDMGFQYSCI NDYRPVKLLQ CVDHSTHPDC ALKSAAAATQ RKAPSLYTEQRAGLIIPLFLVLA

[ REFERENCES ]

1. Kaisho T., et al. (1994) Proc. Natl. Acad. Sci. U.S.A. 91:5325-5329.

2. Liu T., et al. (2005) J. Proteome Res. 4:2070-2080.

3. Chen R., et al. (2009) J. Proteome Res. 8:651-661.

4. Yamamoto-Katayama S., et al. (2002) J. Mol. Biol. 316:711-723.

[ IDENTIFICATION ]

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