糖蛋白130(gp130)重组蛋白(Human,人2)

Recombinant Glycoprotein 130 (gp130)

CDw130; CD130; IL6ST; GP130-RAPS; IR6RB; IL6R-Beta; IR6RB; IR6-RB; Interleukin 6 Signal Transducer; Oncostatin M Receptor; Interleukin-6 Receptor Subunit Beta

[ PROPERTIES ]

Source: Prokaryotic expression

Host: E.coli

Residues: Ser606~Gln756

Tags: N-terminal His Tag

Tissue Specificity: Liver Placenta.

Subcellular Location: Cell membrane; Single-pass type I membrane protein.

Purity: >95%

Traits: Freeze-dried powder

Buffer formulation: 10mM PBS, pH7.4, containing 1mMDTT, 5%trehalose, 0.01% sarcosyl and Proclin300.

Original Concentration: 200μg/mL

Applications: Positive Control; Immunogen; SDS-PAGE; WB.

(May be suitable for use in other assays to be determined by the end user.)

Predicted isoelectric point: 5.6

Predicted Molecular Mass: 17.9kDa

Accurate Molecular Mass: 21kDa as determined by SDS-PAGE reducingconditions.

Phenomenon explanation: 

The possible reasons that the actual band size differs fromthe predictedareasfollows:

1.Splice variants: Alternative splicing may create different sized proteins fromthe samegene.

2. Relative charge: The composition of amino acids may affects the charge of the protein.

3. Post-translational modification: Phosphorylation, glycosylation, methylation etc.

4. Post-translation cleavage: Many proteins are synthesized as pro-proteins, and then cleavedtogive the active form.

5. Polymerization of the target protein: Dimerization, multimerization etc.

[ USAGE ]

Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Donotvortex.

[ STORAGE AND STABILITY ]

Storage:Avoid repeated freeze/thaw cycles.

Store at 2-8°C for one month.

Aliquot and store at -80°C for 12 months.

Stability Test: The thermal stability is described by the loss rate. Thelossratewas determined by accelerated thermal degradation test, that is, incubatetheprotein at 37°C for 48h, and no obvious degradation and precipitationwereobserved. The loss rate is less than 5% within the expiration dateunderappropriate storage condition.

[ SEQUENCE ]

[ IDENTIFICATION ]

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