玻连蛋白(VTN)重组蛋白(Mouse,小鼠) 

Recombinant Vitronectin (VTN)

V75; VN; VNT; Serum Spreading Factor; Somatomedin B; Complement S-Protein

[ PROPERTIES ]

Source: Prokaryotic expression

Host: E.coli

Residues: Pro250~Lys478

Tags: N-terminal His Tag

Tissue Specificity: Plasma.

Subcellular Location: Secreted, extracellular space.

Purity: >95%

Traits: Freeze-dried powder

Buffer formulation: 20mM Tris, 150mM NaCl, pH8.0, containing 1mMEDTA, 1mM DTT, 0.01% sarcosyl, 5%Trehalose and Proclin300.

Original Concentration: 200ug/mL

Applications: SDS-PAGE; WB; ELISA; IP; CoIP; Purification; Amine ReactiveLabeling.

(May be suitable for use in other assays to be determined by the end user.)

Predicted isoelectric point: 9.7

Predicted Molecular Mass: 30.5kDa

Accurate Molecular Mass: 41kDa as determined by SDS-PAGE reducingconditions.

Phenomenon explanation: 

The possible reasons that the actual band size differs from the predicted are as follows:

1.Splice variants: Alternative splicing may create different sized proteins from the same gene.

2. Relative charge: The composition of amino acids may affects the charge of the protein.

3. Post-translational modification: Phosphorylation, glycosylation, methylation etc.

4. Post-translation cleavage: Many proteins are synthesized as pro-proteins, and then cleavedto give the active form.

5. Polymerization of the target protein: Dimerization, multimerization etc.

[ USAGE ]

Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0mg/mL. Do not vortex.

[ STORAGE AND STABILITY ]

Storage:Avoid repeated freeze/thaw cycles.

Store at 2-8°C for one month.

Aliquot and store at -80°C for 12 months.

Stability Test: The thermal stability is described by the loss rate. Thelossratewas determined by accelerated thermal degradation test, that is, incubatetheprotein at 37°C for 48h, and no obvious degradation and precipitationwereobserved.The loss rate is less than 5% within the expiration dateunderappropriate storage condition.

[ SEQUENCE ]

[ IDENTIFICATION ]

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