内皮素转化酶1(ECE1)重组蛋白(Mouse,小鼠) 

Recombinant Endothelin Converting Enzyme 1 (ECE1)

[ PROPERTIES ]

Host: E.coli

Residues: Ala326~Asn577,with two N-terminal Tags, His-tag and T7-tag.

Subcellular Location: Cell membrane;Single-pass type II membrane protein.

Purity: >95% 

Traits: Freeze-dried powder

Endotoxin Level: <1.0EU per 1μg(determined by the LAL method).

Applications: SDS-PAGE; WB; ELISA; IP.

(May be suitable for use in other assays to be determined by the end user.)

Predicted isoelectric point: 6.2

Predicted Molecular Mass: 33.3kDa

[ USAGE ]

Reconstitute in sterile PBS, pH7.2-pH7.4.

[ STORAGE AND STABILITY ]

Storage:Avoid repeated freeze/thaw cycles.

Store at 2-8°C for one month.

Aliquot and store at -80°C for 12 months.

Stability Test: The thermal stability is described by the loss rate of thetargetprotein. The loss rate was determined by accelerated thermal degradationtest,that is, incubate the protein at 37°C for 48h, and no obvious degradationandprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this proteinislessthan 5% within the expiration date under appropriate storage condition.

[ SEQUENCES ]

The target protein is fused with two N-terminal Tags, His-tag and T7-tag, itssequence is listed below.

MGSSHHHHHH SSG LVPRGSH MASMTGGQQM GRGSEF -APAIN WLPFLNTIFYPVEINESEPI VVYDKEYLRQ VSTLINNTDK CLLNNYMMWN LVRKTSSFLD QRFQDADEKFMEVMYGTKKT CIPRWKFCVS DTENNLGFAL GPMFVKATFA EDSKNIASEI IMEIKKAFEESLSTLKWMDE ETRRSAKEKA DAIYNMIGYP NFIMDPKELD KVFNDYTAVP DLYFENAMRFFNFSWRVTAD QLRKAPNRDQ WSMTPPMVNA YYSPTKNEIV FPAGILQAPF YTRSSPN

[ IDENTIFICATION ]

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