产品展厅
产品展厅
微管关联蛋白4(MAP4)重组蛋白 (Mouse,小鼠)
Recombinant Microtubule Associated Protein 4 (MAP4)
[ PROPERTIES ]
Source: Prokaryotic expression
Host: E.coli
Residues: Thr882~Glu1062
Tags: N-terminal His Tag
Tissue Specificity: Kidney, Brain.
Subcellular Location: Cytoplasm, cytoskeleton.
Purity: >95%
Traits: Freeze-dried powder
Buffer formulation: 100mM NaHCO3, 500mM NaCl, pH8.3, containing1mMEDTA, 1mM DTT, 0.01% sarcosyl, 5%Trehalose and Proclin300.
Original Concentration: 200ug/mL
Applications: SDS-PAGE; WB; ELISA; IP; CoIP; Purification; Amine ReactiveLabeling.
(May be suitable for use in other assays to be determined by the end user.)
Predicted isoelectric point: 10.2
Predicted Molecular Mass: 22.3kDa
Accurate Molecular Mass: 27kDa as determined by SDS-PAGE reducingconditions.
Phenomenon explanation:
The possible reasons that the actual band size differs from the predicted are as follows:
1.Splice variants: Alternative splicing may create different sized proteins from the same gene.
2. Relative charge: The composition of amino acids may affects the charge of the protein.
3. Post-translational modification: Phosphorylation, glycosylation, methylation etc.
4. Post-translation cleavage: Many proteins are synthesized as pro-proteins, and then cleavedto give the active form.
5. Polymerization of the target protein: Dimerization, multimerization etc.
[ USAGE ]
Reconstitute in 100mM NaHCO3, 500mM NaCl (pH8.3) to a concentrationof0.1-1.0 mg/mL. Do not vortex.
[ STORAGE AND STABILITY ]
Storage:Avoid repeated freeze/thaw cycles.
Store at 2-8°C for one month.
Aliquot and store at -80°C for 12 months.
Stability Test: The thermal stability is described by the loss rate. Thelossratewas determined by accelerated thermal degradation test, that is, incubatetheprotein at 37°C for 48h, and no obvious degradation and precipitationwereobserved. The loss rate is less than 5% within the expiration dateunderappropriate storage condition.
[ SEQUENCE ]
[ IDENTIFICATION ]
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