信号调节蛋白β1(SIRPb1)重组蛋白(Human,人) 

Recombinant Signal Regulatory Protein Beta 1 (SIRPb1)

CD172-B; CD172b; SIRP-B1; SIRP-beta-1; CD172 antigen-like family member B

[ PROPERTIES ]

Source: Prokaryotic expression

Host: E.coli

Residues: Ser127~Gly356

Tags: N-terminal His-Tag

Tissue Specificity: Skin.

Subcellular Location: Membrane; Single-pass type I membrane protein.

Purity: > 95%

Traits: Freeze-dried powder

Buffer formulation: 100mM NaHCO?, 500mM NaCl, pH8.3, containing1mMEDTA, 1mM DTT, 0.01% sarcosyl, 5%Trehalose and Proclin300.

Original Concentration: 200μg/mL

Applications: SDS-PAGE; WB; ELISA; IP; CoIP; Purification; Amine ReactiveLabeling.

(May be suitable for use in other assays to be determined by the end user.)

Predicted isoelectric point: 6.0

Predicted Molecular Mass: 28.9kDa

Accurate Molecular Mass: 32kDa as determined by SDS-PAGE reducingconditions.

Phenomenon explanation:

The possible reasons that the actual band size differs from the predicted are as follows:

1.Splice variants: Alternative splicing may create different sized proteins from the same gene.

2. Relative charge: The composition of amino acids may affects the charge of the protein.

3. Post-translational modification: Phosphorylation, glycosylation, methylation etc.

4. Post-translation cleavage: Many proteins are synthesized as pro-proteins, and then cleavedto give the active form.

5. Polymerization of the target protein: Dimerization, multimerization etc.

[ USAGE ]

Reconstitute in 100mM NaHCO?, 500mM NaCl (pH8.3) to a concentrationof0.1-1.0 mg/mL. Do not vortex.

[ STORAGE AND STABILITY ]

Storage: Avoid repeated freeze/thaw cycles.

Store at 2-8°C for one month.

Aliquot and store at -80°C for 12 months.

Stability Test: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

[ SEQUENCE ]

[ IDENTIFICATION ]

[ IMPORTANT NOTE ]

The kit is designed for research use only, we will not be responsible for any issue if the kit wasused in clinical diagnostic or any other procedures.

特别提示:本公司的所有产品仅可用于科研实验,严禁用于临床医疗及其他非科研用途!以实际收货产品说明书为准,网站说明书仅供参考。