肌醇六磷酸激酶3(IHPK3)重组蛋白(Human,人)

Recombinant Inositol Hexaphosphate Kinase 3 (IHPK3)

IP6K3; INSP6K3

[ PROPERTIES ]

Host: E.coli

Residues: Ala154~Glu410

Tags: Two N-terminal Tags, His-tag and T7-tag

Subcellular Location: Cytoplasm.

Purity: >95%

Endotoxin Level: <1.0EU per 1μg (determined by the LAL method).

formulation: Supplied as lyophilized form in 20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% sarcosyl, 5% trehalose, and preservative.

Applications: SDS-PAGE; WB; ELISA; IP.

(May be suitable for use in other assays to be determined by the end user.)

Predicted isoelectric point: 8.2

Predicted Molecular Mass: 33.2kDa

[ USAGE ]

Reconstitute in sterile ddH₂O.

[ STORAGE AND STABILITY ]

Storage:Avoid repeated freeze/thaw cycles.

Store at 2-8°C for one month.

Aliquot and store at -80°C for 12 months.

Stability Test: The thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. (Referring from China Biological Products Standard, which was calculated by the Arrhenius equation.) The loss of this protein is less than 5% within the expiration date under appropriate storage condition.

[ SEQUENCES ]

The sequence of the target protein is listed below.

AFSLVED TNGNQVERKS FNPWGLQCHQ AHLTRLCSEY PENKRHRFLL LENVVSQYTH

PCV L D LKMG T RQH GD DAS EE KKA R HM RK CA QST SA C LGV R IC GMQVYQ T D

KKYFLCKDKY YGRKLSVEGF RQALYQFLHN GSHLRRELLE PILHQLRALL SVIRSQSSYR

FYSSSLLVIY DGQEPPERAP GSPHPHEAPQ AAHGSSPGGL TKVDIRMIDF AHTTYKGYWN

EHTTYDGPDP GYIFGLENLI RILQDIQEGE

[ IDENTIFICATION ]

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