内皮糖蛋白(ENG)重组蛋白 (Human,人)  

Recombinant Endoglin (ENG)

CD105; END; HHT1; ORW; ORW1; Osler-Rendu-Weber Syndrome 1

[ DESCRIPTION ]

Protein Names: Endoglin

(The proteins are labeled by biotin.)

Synonyms: ENG, END

Species: Human

Size: 10μg

Source: Escherichia coli-derived

Subcellular Location: Membrane; Single-pass type I membrane protein.

[ PROPERTIES ]

Residues: Thr336~Phe450,with N-terminal His-Tag. 

Grade & Purity: >90%, 16kDa as determined by SDS-PAGE reducing conditions.

Formulation: Supplied as liquid form in PBS, pH 7.4,containing 50% glycerol.

Endotoxin Level: <1.0 EU per 1μg (determined by the LAL method).

Applications: SDS-PAGE; WB; ELISA; IP.

(May be suitable for use in other assays to be determined by the end user.)

Predicted Molecular Mass: 14.0kDa

Predicted isoelectric point: 6.4

[ PREPARATION ]

Reconstitute in sterile PBS, pH7.2-pH7.4.

[ STORAGE AND STABILITY ]

Storage: Avoid repeated freeze/thaw cycles.

Store at 2-8℃ for one month.

Aliquot and store at -80℃ for 12 months.

Stability Stability Stability Stability Test: The thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. (Referring from China Biological Products Standard, which was calculated by the Arrhenius equation.) The loss of this protein is less than 5% within the expiration date under appropriate storage condition.

[ SEQUENCES ]

The target protein is fused with N-terminal His-Tag, its sequence is listed below.

MGHHHHHHSGSEF-TSPAP IQTTPPKDTC SPELLMSLIQ TKCADDAMTL VLKKELVAHL

KCTITGLTFW DPSCEAEDRG DKFVLRSAYS SCGMQVSASM ISNEAVVNIL SSSSPQRKKV

HCLNMDSLSF

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