沉默调节蛋白6(SIRT6)重组蛋白(Mouse,小鼠)  

Recombinant Sirtuin 6 (SIRT6)

SIR2L6; Silent Mating Type Information Regulation 2 Homolog 6; Mono-ADP-Ribosyltransferase Sirtuin-6; Regulatory protein SIR2 homolog 6; SIR2-like protein 6

[ PROPERTIES ]

Residues: Met1~Ser334,with N-terminal His-Tag.

Host: E. coli

Subcellular Location: Nucleus.

Purity: >95%

Endotoxin Level: <1.0EU per 1μg

(determined by the LAL method).

Formulation: Supplied as lyophilized form in PBS,pH7.4, containing 5% sucrose, 0.01% sarcosyl.

Predicted isoelectric point: 8.9

Predicted Molecular Mass: 38.4kDa

Applications: SDS-PAGE; WB; ELISA; IP.

(May be suitable for use in other assays to be determined by the end user.)

[ USAGE ]

Reconstitute in sterile PBS, pH7.2-pH7.4.

[ STORAGE AND STABILITY ]

Storage: Avoid repeated freeze/thaw cycles.

Store at 2-8℃ for one month.

Aliquot and store at -80℃ for 12 months.

Stability Test: The thermal stability is described by the loss rate of thetargetprotein. The loss rate was determined by accelerated thermal degradationtest,that is, incubate the protein at 37℃ for 48h, and no obvious degradationandprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this proteinislessthan 5% within the expiration date under appropriate storage condition.

[ SEQUENCES ]

The target protein is fused with N-terminal His-Tag, its sequence is listedbelow.

MGHHHHHHSGSEF-MSVNYAAGLS PYADKGKCGL PEIFDPPEEL ERKVWELARLMWQSSSVVFH TGAGISTASG IPDFRGPHGV WTMEERGLAP KFDTTFENAR PSKTHMALVQLE R MG F LS F L VS Q NV D G L HV RS G F P R DK L A EL H G NM F V E E CP K CK T Q Y V RDTVVGTMGLK ATGRLCTVAK TRGLRACRGE LRDTILDWED SLPDRDLMLA DEASRTADLSVTLGTSLQIR PSGNLPLATK RRGGRLVIVN LQPTKHDRQA DLRIHGYVDE VMCRLMKHLGLEIPAWDGPC VLDKALPPLP RPVALKAEPP VHLNGAVHVS YKSKPNSPIL HRPPKRVKTEAAPS

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