单核细胞趋化蛋白3(MCP3)重组蛋白(Rat,大鼠) 

Recombinant Monocyte Chemotactic Protein 3 (MCP3)

CCL7; FIC; MARC; NC28; MCP3; SCYA7; Chemokine C-C-Motif Ligand 7; Small Inducible Cytokine A7; Monocyte Chemoattractant Protein 3

[ PROPERTIES ]

Host: E.coli

Residues: Gln24~Pro97 , with two N-terminal Tags, His-tag and T7-tag

Subcellular Location: Secreted

Purity: >95% 

Endotoxin Level: <1.0EU per 1μg (determined by the LAL method).

formulation: Supplied as lyophilized form in PBS, pH7.4, containing 5% trehalose, 0.01% sarcosyl.

Applications: SDS-PAGE; WB; ELISA; IP.

(May be suitable for use in other assays to be determined by the end user.)

Predicted isoelectric point: 9.7

Predicted Molecular Mass: 12.3kDa

[ USAGE ]

Reconstitute in sterile PBS, pH7.2-pH7.4.

[ STORAGE AND STABILITY ]

Storage:Avoid repeated freeze/thaw cycles.

Store at 2-8°C for one month.

Aliquot and store at -80°C for 12 months.

Stability Test: The thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is less than 5% within the expiration date under appropriate storage condition.

[ SEQUENCES ]

The target protein is fused with two N-terminal Tags, His-tag and T7-tag, its sequence is listed below.

MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF- QPDGTNS STCCYVKKQK

IPKRNLKSYR KITSSRCPWE AVIFKTKKGM EVCAEAHQKW VEEAIAYLDM KTSTPKP

[ IDENTIFICATION ]

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