L-型电压依赖钙离子通道α1S亚基(CACNa1S)重组蛋白 (Rat,大鼠)
Recombinant Calcium Channel, Voltage Dependent, L-Type, Alpha 1S Subunit (CACNa1S)
CACNL1A3; CCHL1A3; Cav1.1; HOKPP; MHS5; hypoPP; DHPR; Dihydropyridine Receptor; Voltage-gated calcium channel subunit alpha Cav1.1
[ PROPERTIES ]
Accession: Q02485
Host: E.coli
Residues: Gln1554~Ser1767
Tags: Two N-terminal Tags, His-tag and T7-tag
Subcellular Location: Cell Membrane. Multi-passMembrane Protein.
Purity: >90%
Endotoxin Level: <1.0EU per 1μg(determined by the LAL method).
Formulation: Supplied as lyophilized form in 20mM Tris,150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT,0.01% sarcosyl, 5% trehalose, and preservative.
Applications: SDS-PAGE; WB; ELISA; IP.
(May be suitable for use in other assays to be determined by the end user.)
Predicted isoelectric point: 5.1
Predicted Molecular Mass: 27.6kDa
[ USAGE ]
Reconstitute in sterile ddH?O.
[ STORAGE AND STABILITY ]
Storage:Avoid repeated freeze/thaw cycles.
Store at 2-8°C for one month.
Aliquot and store at -80°C for 12 months.
Stability Test: The thermal stability is described by the loss rate of thetargetprotein. The loss rate was determined by accelerated thermal degradationtest,that is, incubate the protein at 37°C for 48h, and no obvious degradationandprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this proteinislessthan 5% within the expiration date under appropriate storage condition.
[ SEQUENCES ]
The sequence of the target protein is listed below.
QIQAGLR TIEEEAAPEI HRAISGDLTA EEELERAMVE AAMEEGIFRR TGGLFGQVDNFLERTNSLPP VMANQRPLQF AEMEMEELES PVFLEDFPQN PGTHPLARAN TNNANANVAYGN S S H R N S P V FS SI RY E R E L LE E A G R P V T R EG P F S Q P C S V SG V NS RS HV DKLERQMSQRR MPKGQVPPSP CQLSQEKHPV HEEGKGPRSW STETSDS
[ IDENTIFICATION ]

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