抑制素βB(INHbB)重组蛋白 (Gallus,鸡)
Recombinant Inhibin Beta B (INHbB)
INH-BB; Activin AB Beta Polypeptide
[ PROPERTIES ]
Host: E.coli
Residues: Gly277~Ala391 (Accession # P27093),with N-terminal His-Tag
Subcellular Location: Secreted
Purity: >95%
Endotoxin Level: <1.0EU per 1μg(determined by the LAL method).
formulation: Supplied as lyophilized form in PBS,pH7.4, containing 5% sucrose, 0.01% sarcosyl.
Predicted isoelectric point: 5.9
Predicted Molecular Mass: 14.3kDa
Applications: SDS-PAGE; WB; ELISA; IP.
(May be suitable for use in other assays to be determined by the end user.)
[ USAGE ]
Reconstitute in sterile PBS, pH7.2-pH7.4.
[ STORAGE AND STABILITY ]
Storage:Avoid repeated freeze/thaw cycles.
Store at 2-8°C for one month.
Aliquot and store at -80°C for 12 months.
Stability Test: The thermal stability is described by the loss rate of thetargetprotein. The loss rate was determined by accelerated thermal degradationtest,that is, incubate the protein at 37°C for 48h, and no obvious degradationandprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this proteinislessthan 5% within the expiration date under appropriate storage condition.
[ SEQUENCES ]
The target protein is fused with N-terminal His-Tag, its sequence is listedbelow.
MGHHHHHHSGSEF-GLEC DGRTNLCCRQ QFYIDFRLIG WNDWIIAPSGYYGNYCEGSCPAYLAGVPGS ASSFHTAVVN QYRMRGLNPG TVNSCCIPTK LSTMSMLYFD DEYNIVKRDVPNMIVEECGC A
[ IDENTIFICATION ]

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