表皮转谷氨酰胺酶(TGM3)重组蛋白 (Human,人) 

Recombinant Transglutaminase 3, Epidermal (TGM3)

TGE; Egt; Protein-Glutamine Gamma-Glutamyltransferase E; Transglutaminase E

[ DESCRIPTION ]

Protein Names: Transglutaminase 3, Epidermal

Synonyms: TGM3

Species: Human

Size: 10μg

Source: Escherichia coli-derived

[ PROPERTIES ]

Residues: Ala468~Glu693 ,with N-terminal His-Tag.

Grade & Purity: >95%, 28kDa as determined bySDS-PAGE reducing conditions.

Formulation: Supplied as lyophilized form in PBS, pH7.4, containing 5% sucrose.

Endotoxin Level: <1.0 EU per 1μg (determined bythe LAL method).

Applications: SDS-PAGE; WB; ELISA; IP.

(May be suitable for use in other assays to be determined by the end user.)

Predicted isoelectric point: 5.5

Predicted Molecular Mass: 26.3kDa

[ PREPARATION ]

Reconstitute in sterile PBS, pH7.2-pH7.4.

[ STORAGE AND STABILITY ]

Storage:Avoid repeated freeze/thaw cycles.

Store at 2-8°C for one month.

Aliquot and store at -80°C for 12 months.

Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37°C for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.

[ SEQUENCES ]

The target protein is fused with N-terminal His-Tag, its sequence is listed below.MGHHHHHHSGS- ATS SMGLETEEQE PSIIGKLKVA GMLAVGKEVN LVLLLKNLSRDTKTVTVNMT AWTIIYNGTL VHEVWKDSAT MSLDPEEEAE HPIKISYAQY EKYLKSDNMIRITAVCKVPD ESEVVVERDI ILDNPTLTLE VLNEARVRKP VNVQMLFSNP LDEPVRDCVLMVEGSGLLLG NLKIDVPTLG PKEGSRVRFD ILPSRSGTKQ LLADFSCNKF PAIKAMLSIDVAE

[ REFERENCES ]

1. Liu J., et al. (2012) Lin Chung Er Bi Yan Hou Tou Jing Wai Ke Za Zhi26:101-103.

2. Zone J.J., et al. (2011) J. Immunol. 186:4474-4480.

3. Burkard T.R., et al. (2011) BMC Syst. Biol. 5:17-17.

4. Yamane A., et al. (2010) FEBS J. 277:3564-3574.

[ IDENTIFICATION ]

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