穿孔素1(PRF1)重组蛋白(Rat,大鼠) 

Recombinant Perforin 1 (PRF1)

FLH2; HPLH2; P1; PFP; Pore Forming Protein; Cytolysin; Lymphocyte pore-forming protein

[ PROPERTIES ]

Host: E.coli

Residues: Ala120~Asp353,with two N-terminal Tags, His-tag and T7-tag.

Subcellular Location: Cytoplasmic granulelumen.Secreted.Cell membrane; Multi-passmembrane protein. Endosome lumen.

Purity: >95%

Endotoxin Level: <1.0EU per 1μg(determined by the LAL method).

Formulation: Supplied as lyophilized form in PBS,pH7.4, containing 5% trehalose, 0.01% sarcosyl.

Applications: SDS-PAGE; WB; ELISA; IP.

(May be suitable for use in other assays to be determined by the end user.)

Predicted isoelectric point: 6.3

Predicted Molecular Mass: 29.7kDa

[ USAGE ]

Reconstitute in sterile PBS, pH7.2-pH7.4.

[ STORAGE AND STABILITY ]

Storage:Avoid repeated freeze/thaw cycles.

Store at 2-8°C for one month.

Aliquot and store at -80°C for 12 months.

Stability Test: The thermal stability is described by the loss rate of thetargetprotein. The loss rate was determined by accelerated thermal degradationtest,that is, incubate the protein at 37°C for 48h, and no obvious degradationandprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this proteinislessthan 5% within the expiration date under appropriate storage condition.

[ SEQUENCES ]

The target protein is fused with two N-terminal Tags, His-tag and T7-tag, itssequence is listed below.

MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF- A AANINNDWRA GLDVNPKPEANVHVSVAGSH SKIANFAAEK AHQDQYNFNT DTVECRMYSF RLAQKPPLHP DFRKALKNLPHNFNSSTEHA YRRLISSYGT HFITAVDLGG RVSVLTALRT CQLTLDGLTA DEVGDCLSVEAQVSIGAQAS VSSEYKACEE KKKQHKIATS FHQTYRERHV EVLGGPLDSS NDLLFGNQATPEHFSTWIAS LPTRPDVVDY SLEPLHILLE DSD

[ IDENTIFICATION ]

[ IMPORTANT NOTE ]

The kit is designed for research use only, we will not be responsible for any issue if the kit wasused in clinical diagnostic or any other procedures.

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