N-甲基嘌呤DNA糖基化酶(MPG)重组蛋白(Mouse,小鼠)

Recombinant N-Methylpurine DNA Glycosylase (MPG)

AAG; APNG; ADPG; CRA36.1; MDG; Mid1; PIG11; PIG16; Anpg; DNA-3-Methyladenine Glycosylase; Alkyladenine DNA Glycosylase; 3-alkyladenine DNA glycosylase

[ PROPERTIES ]

Host: E.coli

Residues: Leu46~Gln315 ,with N-terminal His-Tag.

Subcellular Location: Nucleus.

Purity: >95% 

Endotoxin Level: <1.0EU per 1μg(determined by the LAL method).

Formulation: Supplied as lyophilized form in PBS,pH7.4, containing 5% sucrose, 0.01% sarcosyl.

Applications: SDS-PAGE; WB; ELISA; IP.

(May be suitable for use in other assays to be determined by the end user.)

Predicted isoelectric point: 8.9

Predicted Molecular Mass: 31.4kDa

[ USAGE ]

Reconstitute in sterile PBS, pH7.2-pH7.4.

[ STORAGE AND STABILITY ]

Storage:Avoid repeated freeze/thaw cycles.

Store at 2-8°C for one month.

Aliquot and store at -80°C for 12 months.

Stability Test: The thermal stability is described by the loss rate of thetargetprotein. The loss rate was determined by accelerated thermal degradationtest,that is, incubate the protein at 37°C for 48h, and no obvious degradationandprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this proteinislessthan 5% within the expiration date under appropriate storage condition.

[ SEQUENCES ]

The target protein is fused with N-terminal His-Tag, its sequence is listedbelow.

MGHHHHHHSG SEF-LVTGY HEVGQMPAPL SRKIGQKKQR LADSEQQQTP KERLLSTPGLRRSIYFSSPE DHSGRLGPEF FDQPAVTLAR AFLGQVLVRR LADGTELRGRIVETEAYLGPEDEAAHSRGG RQTPRNRGMF MKPGTLYVYL IYGMYFCLNV SSQGAGACVL LRALEPLEGLETMRQLRNSL RKSTVGRSLK DRELCSGPSK LCQALAIDKS FDQRDLAQDD AVWLEHGPLESSSPAVVVAAARIGIGHAGE WTQKPLRFYV QGSPWVSVVD RVAEQ

[ IDENTIFICATION ]

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