β-位淀粉样前体蛋白裂解酶1(bACE1)重组蛋白(Mouse,小鼠) 

Recombinant Beta-Site APP Cleaving Enzyme 1 (bACE1)

ASP2; Beta Secretase; Memapsin 2; Aspartyl protease 2; Beta-site amyloid precursor protein cleaving enzyme 1; Membrane-associated aspartic protease 2

[ PROPERTIES ]

Source: Prokaryotic expression

Host: E.coli

Residues: Pro190~Ala430 ,with two N-terminal Tags, His-tag and GST-tag.

Tags: N-terminal His Tag

Subcellular Location: Membrane; Single-passtype I membrane protein. Golgi apparatus,trans-Golgi network. Endoplasmic reticulum.Endosome. Cell surface. Cytoplasmic vesiclemembrane

Purity: >95%

Endotoxin Level: <1.0EU per 1μg(determined by the LAL method).

Formulation: SDS-PAGE; WB; ELISA; IP.

(May be suitable for use in other assays to be determined by the end user.)

Predicted isoelectric point: 5.8

Predicted Molecular Mass: 59.0kDa

[ USAGE ]

Reconstitute in sterile PBS, pH7.2-pH7.4.

[ STORAGE AND STABILITY ]

Storage:Avoid repeated freeze/thaw cycles.

Store at 2-8°C for one month.

Aliquot and store at -80°C for 12 months.

Stability Test: The thermal stability is described by the loss rate of thetargetprotein. The loss rate was determined by accelerated thermal degradationtest,that is, incubate the protein at 37°C for 48h, and no obvious degradationandprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this proteinislessthan 5% within the expiration date under appropriate storage condition.

[ SEQUENCES ]

The target protein is fused with two N-terminal Tags, His-tag and GST-tag, itssequence is listed below.

MSPILGYWKI KGLVQPTRLL LEYLEEKYEE HLYERDEGDK WRNKKFELGL EFPNLPYYIDGDVKLTQSMA IIRYIADKHN MLGGCPKERA EISMLEGAVL DIRYGVSRIA YSKDFETLKVDFLSKLPEML KMFEDRLCHK TYLNGDHVTH PDFMLYDALD VVLYMDPMCL DAFPKLVCFKKRIEAIPQID KYLKSSKYIA WPLQGWQATF GGGDHPPKSD GSTSGSGHHH HHHSAGLVPRGSTAIGMKET AAAKFERQHM DSPDLGTLEV LFQGPLGSEF- P DDSLEPFFDS LVKQTHIPNIFSLQLCGAGF PLNQTEALAS VGGSMIIGGI DHSLYTGSLW YTPIRREWYY EVIIVRVEINGQDLKMDCKE YNYDKSIVDS GTTNLRLPKK VFEAAVKSIK AASSTEKFPD GFWLGEQLVCWQAGTTPWNI FPVISLYLMG EVTNQSFRIT ILPQQYLRPV EDVATSQDDC YKFAVSQSSTGTVMGAVIME GFYVVFDRAR KRIGFAVSAC HVHDEFRTAA

[ IDENTIFICATION ]

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