过氧化物酶体增殖物激活受体δ(PPARd)重组蛋白 (Mouse,小鼠) 

Recombinant Peroxisome Proliferator Activated Receptor Delta (PPARd)

FAAR; NR1C2; NUC1; NUCI; PPARB; PPARd/B; Nuclear Receptor Subfamily 1,Group C,Member 2; Nuclear hormone receptor 1; Peroxisome proliferator-activated receptor beta

[ PROPERTIES ]

Source: Prokaryotic expression

Host: E.coli

Residues: Asn29~Met147

Tags: N-terminal His Tag

Tissue Specificity: Heart, Liver, Brain.

Subcellular Location: Nucleus.

Purity: >92% 

Traits: Freeze-dried powder

Buffer formulation: 20mM Tris, 150mM NaCl, pH8.0, containing 1mMEDTA, 1mM DTT, 0.01% sarcosyl, 5%Trehalose and Proclin300.

Original Concentration: 200μg/mL

Applications: Positive Control; Immunogen; SDS-PAGE; WB.

(May be suitable for use in other assays to be determined by the end user.)

Predicted isoelectric point: 9.1

Predicted Molecular Mass: 14.5kDa

Accurate Molecular Mass: 18&19&20kDa as determined by SDS-PAGEreducingconditions.

Phenomenon explanation: 

The possible reasons that the actual band size differs fromthe predictedareasfollows:

1.Splice variants: Alternative splicing may create different sized proteins from the same gene.

2. Relative charge: The composition of amino acids may affects the charge of the protein.

3. Post-translational modification: Phosphorylation, glycosylation, methylation etc.

4. Post-translation cleavage: Many proteins are synthesized as pro-proteins, and then cleavedto give the active form.

5. Polymerization of the target protein: Dimerization, multimerization etc.

[ USAGE ]

Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0mg/mL. Do not vortex

[ STORAGE AND STABILITY ]

Storage:Avoid repeated freeze/thaw cycles.

Store at 2-8°C for one month.

Aliquot and store at -80°C for 12 months.

Stability Test: The thermal stability is described by the loss rate. The loss rate was determinedby accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and noobvious degradation and precipitation were observed. The loss rate is less than 5% within theexpiration date under appropriate storage condition.

[ SEQUENCE ]

[ IDENTIFICATION ]

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