对氧磷酶1(PON1)重组蛋白(Mouse,小鼠) 

Recombinant Paraoxonase 1 (PON1)

ESA; PON; Esterase A; Serum paraoxonase/arylesterase 1; Aromatic esterase 1; A-esterase 1; Serum aryldialkylphosphatase 1

[ PROPERTIES ]

Source: Prokaryotic expression

Host: E.coli

Residues: Ala187~Asp354,with N-terminal His-Tag

Tags: N-terminal His Tag

Subcellular Location: Secreted, extracellularspace.

Purity: >95%

Endotoxin Level: <1.0EU per 1μg(determined by the LAL method).

Formulation: Supplied as lyophilized form in PBS,pH7.4, containing 1mM DTT, 5% trehalose, 0.05% sarcosyl and preservative.

Applications: SDS-PAGE; WB; ELISA; IP.

(May be suitable for use in other assays to be determined by the end user.)

Predicted isoelectric point: 5.7

Predicted Molecular Mass: 20.3kDa

Accurate Molecular Mass: 5

[ USAGE ]

Reconstitute in sterile PBS, pH7.2-pH7.4.

[ STORAGE AND STABILITY ]

Storage:Avoid repeated freeze/thaw cycles.

Store at 2-8°C for one month.

Aliquot and store at -80°C for 12 months.

Stability Test: The thermal stability is described by the loss rate of thetargetprotein. The loss rate was determined by accelerated thermal degradationtest,that is, incubate the protein at 37°C for 48h, and no obvious degradationandprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this proteinislessthan 5% within the expiration date under appropriate storage condition.

[ SEQUENCES ]

The target protein is fused with N-terminal His-Tag, its sequence is listedbelow.

MGHHHHHHSGSEF- ADPY LRSWEMYLGL SWSNVVYYSP DKVQVVAEGF DFANGIGISLDGKYVYIAEL LAHKIHVYEK HANWTLTPLK VLNFDTLVDN ISVDPVTGDL WVGCHPNGMRIFFYDAENPP GSEVLRIQNI LSEDPKITVV YAENGTVLQG TTVASVYKGK LLIGTVFHKALYC

[ REFERENCES ]

1. Sorenson R.C., et al. (1995) Proc. Natl. Acad. Sci. U.S.A. 92:7187-7191.

2. Shih D.M., et al. (1996) J. Clin. Invest. 97:1630-1639.

3. Li W.F., et al. (1997) Pharmacogenetics 7:137-144.

4. Yang F., et al. (2005) FEBS Lett. 579:3713-3717.

[ IDENTIFICATION ]

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