CD5抗原样蛋白(CD5L)重组蛋白(Rat,大鼠)
Recombinant CD5 Antigen Like Protein (CD5L)
API6; CT-2; SP-ALPHA; Spalpha; Apoptosis Inhibitor 6; Scavenger Receptor Cysteine Rich Family; IgM-associated peptide
[ PROPERTIES ]
Host: E.coli
Residues: Leu11~Leu346 ,with two N-terminal Tags, His-tag and GST-tag.
Endotoxin Level: <1.0EU per 1μg(determined by the LAL method).
Purity: >95%
Formulation: Supplied as lyophilized form in PBS,pH7.4, containing 5% sucrose, 0.01% sarcosyl.
Applications: SDS-PAGE; WB; ELISA; IP.
(May be suitable for use in other assays to be determined by the end user.)
Predicted isoelectric point: 5.8
Predicted Molecular Mass: 63.8kDa
[ USAGE ]
Reconstitute in sterile PBS, pH7.2-pH7.4.
[ STORAGE AND STABILITY ]
Storage:Avoid repeated freeze/thaw cycles.
Store at 2-8°C for one month.
Aliquot and store at -80°C for 12 months.
Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37°C for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.
[SEQUENCES ]
The target protein is fused with two N-terminal Tags, His-tag and GST-tag, itssequence is listed below.
MRNKKFELGL EFPNLPYYID GDVKLTQSMA IIRYIADKHN MLGGCPKERA EISMLEGAVLDIRYGVSRIA YSKDFETLKV DFLSKLPEML KMFEDRLCHK TYLNGDHVTH PDFMLYDALDVVLYMDPMCL DAFPKLVCFK KRIEAIPQID KYLKSSKYIA WPLQGWQATF GGGDHPPKSDGS T S G S G H H H HH HSA G LVP R GS TA IG MK E T AA AK F E R Q HM DSP D L G T L EVLFQGPLGSEF-LLTIFVGLCF SESPTRVRLV GGAHRCEGRV EVKHNGQWGT VCDDGWDLIDVSVVCRELNC GAAKKTPSGA SYHPPASEGQ SVLIQGVECS GAEDMLAQCT LNYDVFDCSHAEDAGVQCEN PDNPENVRLV EG LGRCQGR L EVFYQGQWST VCKAGWN LQASKVVCRQ LGC GRA L LAH RCC NKNTQGKGPI WMSKMSCSGR EAN LQ DCPFSPLESN CT H GE DTWMECE DPF EL R LV GG DT T CSGR LEV L HK GSWGSV CDDSWGEKE DQVVC KQ LG CGKS L F PSPKA RQSF G PG TG RIW L DD VSCSGKEES LELCRHRLWGY HDCTHKEDVG VICSEL
[ IDENTIFICATION ]

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