白细胞激活黏附因子(ALCAM)重组蛋白(Rabbit,兔) 

Recombinant Activated Leukocyte Cell Adhesion Molecule (ALCAM)

CD166; MEMD; Activated Leucocyte Cell Adhesion Molecule

[ PROPERTIES ]

Host: E.coli

Residues: Gly225~Ala478 ,with N-terminal His-Tag.

Subcellular Location: Membrane; Single-passtype I membrane protein.

Purity: >95% 

Endotoxin Level: <1.0EU per 1μg(determined by the LAL method).

Formulation: Supplied as lyophilized form in PBS,pH7.4, containing 5% sucrose, 0.01% sarcosyl.

Applications: SDS-PAGE; WB; ELISA; IP.

(May be suitable for use in other assays to be determined by the end user.)

Predicted isoelectric point: 6.2

Predicted Molecular Mass: 29.3kDa

[ USAGE ]

Reconstitute in sterile PBS, pH7.2-pH7.4.

[ STORAGE AND STABILITY ]

Storage:Avoid repeated freeze/thaw cycles.

Store at 2-8°C for one month.

Aliquot and store at -80°C for 12 months.

Stability Test: The thermal stability is described by the loss rate of thetargetprotein. The loss rate was determined by accelerated thermal degradationtest,that is, incubate the protein at 37°C for 48h, and no obvious degradationandprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this proteinislessthan 5% within the expiration date under appropriate storage condition.

[ SEQUENCES ]

The target protein is fused with N-terminal His-Tag, its sequence is listedbelow.

MGHHHHHHSGSEF-GQPEGI RSSNTYTLPN VRRNATGNYK CSLIDKKSLI ASTAITVHYLDLSLNPXGEL TKQIGDSLPV SCTISAIRNA TVVWMKDNIK LRSSPSFSSL QYQDAGNYVCETALQEVEGL KKRESLTLIV EVKPQIKMTK KTDPSGLSKT IICHVEGFPK PAIQWTITGSGSVINQTEES PYINGRYYSK IIISPEENVT LTCAAENQLE RTVNSLNVSA ISIPEHDEADEISDENREKV NDQAKLIVGI VVGLLLAA

[ IDENTIFICATION ]

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