载脂蛋白A4(APOA4)重组蛋白(Mouse,小鼠2) 

Recombinant Apolipoprotein A4 (APOA4)

ApoA-IV; ApoAIV; Apo-A4

[ PROPERTIES ]

Host: E.coli

Residues: Leu135~Asn281 ,with two N-terminal Tags, His-tag and S-tag.

Subcellular Location: Secreted

Purity: >95%

Endotoxin Level: <1.0EU per 1μg(determined by the LAL method).

Formulation: Supplied as lyophilized form in PBS,pH7.4, containing 1mM DTT, 5% trehalose, 0.01%sarcosyl and preservative

Applications: SDS-PAGE; WB; ELISA; IP.

(May be suitable for use in other assays to be determined by the end user.)

Predicted isoelectric point: 6.2

Predicted Molecular Mass: 22.5kDa

Accurate Molecular Mass: 26kDa as determined by SDS-PAGE reducingconditions.

Note: The possible reasons that the actual band size differs from the predicted are as follows:

1.Splice variants: Alternative splicing may create different sized proteins fromthe samegene.

2. Relative charge: The composition of amino acids may affects the charge of the protein.

3. Post-translational modification: Phosphorylation, glycosylation, methylation etc.

4. Post-translation cleavage: Many proteins are synthesized as pro-proteins, and then cleavedtogive the active form.

5. Polymerization of the target protein: Dimerization, multimerization etc.

[ USAGE ]

Reconstitute in sterile PBS, pH7.2-pH7.4.

[ STORAGE AND STABILITY ]

Storage:Avoid repeated freeze/thaw cycles.

Store at 2-8°C for one month.

Aliquot and store at -80°C for 12 months.

Stability Test: The thermal stability is described by the loss rate of thetargetprotein. The loss rate was determined by accelerated thermal degradationtest,that is, incubate the protein at 37°C for 48h, and no obvious degradationandprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this proteinislessthan 5% within the expiration date under appropriate storage condition.

[ SEQUENCES ]

The target protein is fused with two N-terminal Tags, His-tag andS-tag, itssequence is listed below.

MHHHHHHSSG LVPRGSGMKE TAAAKFERQH MDSPDLGTDD DDKAMADIGS EF- LKPYAVDLQDQINTQT QEMKLQLTPY IQRMQTTIKE NVDNLHTSMM PLATNLKDKF NRNMEELKGHLTPRANELKA TIDQNLEDLR RSLAPLTVGV QEKLNHQMEG LAFQMKKNAE ELQTKVSAKIDQLQKNLAPL VEDVQSKVKG N

[ IDENTIFICATION ]

[ REFERENCES ]

1. Williams S.C., et al. (1986) Mol. Cell. Biol. 6:3807-3814.

2. Reue K., Leete T.H. (1991) J. Biol. Chem. 266:12715-12721.

3. Puppione D.L., et al. (2006) Biochim. Biophys. Acta 1764:1363-1371.

4. Sauvaget D., et al. (2002) J. Biol. Chem. 277:34540-34548.

特别提示:本公司的所有产品仅可用于科研实验,严禁用于临床医疗及其他非科研用途!以实际收货产品说明书为准,网站说明书仅供参考。