芳香胺-N-乙酰基转移酶(AANAT)重组蛋白(Rat,大鼠) 

Recombinant Arylalkylamine-N-Acetyltransferase (AANAT)

AA-NAT; SNAT; Aralkylamine N-Acetyltransferase; Serotonin Acetyltransferase; Serotonin Acetylase; Serotonin N-Acetyltransferase; Melatonin Rhythm Enzyme

[ PROPERTIES ]

Accession: P26897

Host: E.coli

Residues: Glu22~Gln235

Tags: Two N-terminal Tags, His-tag and T7-tag

Subcellular Location: Membrane. Single-pass type I membrane protein.

Purity: >90% 

Endotoxin Level: <1.0EU per 1μg (determined by the LAL method).

Formulation: Supplied as lyophilized form in 20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% sarcosyl, 5% trehalose, and preservative.

Applications: SDS-PAGE; WB; ELISA; IP.

(May be suitable for use in other assays to be determined by the end user.)

Predicted isoelectric point: 5.5

Predicted Molecular Mass: 28.2kDa

Accurate Molecular Mass: 37kDa as determined by SDS-PAGE reducing conditions.

Phenomenon explanation: 

Note: The possible reasons that the actual band size differs from the predicted are as follows:

1.Splice variants: Alternative splicing may create different sized proteins from the same gene.

2. Relative charge: The composition of amino acids may affects the charge of the protein.

3. Post-translational modification: Phosphorylation, glycosylation, methylation etc.

4. Post-translation cleavage: Many proteins are synthesized as pro-proteins, and then cleavedto give the active form.

5. Polymerization of the target protein: Dimerization, multimerization etc.

[ USAGE ]

Reconstitute in sterile ddH?O.

[ STORAGE AND STABILITY ]

Storage:Avoid repeated freeze/thaw cycles.

Store at 2-8°C for one month.

Aliquot and store at -80°C for 12 months.

Stability Test: The thermal stability is described by the loss rate of the target

protein. The loss rate was determined by accelerated thermal degradation test,

that is, incubate the protein at 37°C for 48h, and no obvious degradation and

precipitation were observed. (Referring from China Biological Products Standard,

which was calculated by the Arrhenius equation.) The loss of this protein is less

than 5% within the expiration date under appropriate storage condition.

[ SEQUENCES ]

The sequence of the target protein is listed below.

ELCLYDPPE VPNATFKALS YKNGTILNCE CKRGFRRLNE LVYMACLGNS WSNNCQCTSN

SHDNSREQVT PQPEGQKEQQ TTDTQKSTQS VYQE NLAGHC REPPPWRHED

TKRIYHFVEG QIVLYTCIQG YKALQRGPAI SICKTVCGEI RWTHPQLTCV DEKEHHQFLA

SEESQGSRNS FPESEASCPT PNTDFSQLTE 

[ IDENTIFICATION ]

特别提示:本公司的所有产品仅可用于科研实验,严禁用于临床医疗及其他非科研用途!以实际收货产品说明书为准,网站说明书仅供参考。