水通道蛋白4(AQP4)重组蛋白(Rat,大鼠) 

Recombinant Aquaporin 4 (AQP4)

HMIWC2; MIWC; WCH4; Mercurial-insensitive water channel

[ PROPERTIES ]

Source: Prokaryotic expression

Host: E.coli

Residues: Asp179~Asp314

Tags: Two N-terminal Tags, His-tag and GST-tag

Tissue Specificity: Brain, Kidney, Lung.

Subcellular Location: Membrane; Multi-pass membrane protein.

Purity: >98%

Traits: Freeze-dried powder

Buffer formulation: 20mM Tris, 150mM NaCl, pH8.0, containing 1mMEDTA, 1mM DTT, 0.01% sarcosyl, 5%Trehalose and Proclin300.

Original Concentration: 200μg/mL

Applications: Positive Control; Immunogen; SDS-PAGE; WB.

(May be suitable for use in other assays to be determined by the end user.)

Predicted isoelectric point: 6.2

Predicted Molecular Mass: 45.0kDa

Accurate Molecular Mass: 45kDa as determined by SDS-PAGE reducingconditions.

[ USAGE ]

Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0mg/mL. Do not vortex.

[ STORAGE AND STABILITY ]

Storage:Avoid repeated freeze/thaw cycles.

Store at 2-8°C for one month.

Aliquot and store at -80°C for 12 months.

Stability Test: The thermal stability is described by the loss rate. Thelossratewas determined by accelerated thermal degradation test, that is, incubatetheprotein at 37°C for 48h, and no obvious degradation and precipitationwereobserved. The loss rate is less than 5% within the expiration dateunderappropriate storage condition.

[ SEQUENCE ]

[ IDENTIFICATION ]

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