纤溶酶原激活物抑制因子2(PAI2)重组蛋白 (Rat,大鼠) 

Recombinant Plasminogen Activator Inhibitor 2 (PAI2)

SERPINB2; PLANH2; Serpin Peptidase Inhibitor Clade B Member 2; Monocyte Arg-serpin; Placental plasminogen activator inhibitor; Serpin B2; Urokinase inhibitor

[ PROPERTIES ]

Source: Prokaryotic expression

Host: E.coli

Residues: Pro154~Leu408 ,with two N-terminal Tags, His-tag and T7-tag.

Tags: N-terminal His Tag

Subcellular Location: Cytoplasm. Secreted,extracellular space.

Purity: >95% 

Endotoxin Level: <1.0EU per 1μg(determined by the LAL method).

Formulation: Supplied as lyophilized form in PBS,pH7.4, containing 5% sucrose, 0.01% sarcosyl.

Applications: SDS-PAGE; WB; ELISA; IP.

(May be suitable for use in other assays to be determined by the end user.)

Predicted isoelectric point: 5.7

Predicted Molecular Mass: 32.9kDa

[ USAGE ]

Reconstitute in sterile PBS, pH7.2-pH7.4.

[ STORAGE AND STABILITY ]

Storage:Avoid repeated freeze/thaw cycles.

Store at 2-8°C for one month.

Aliquot and store at -80°C for 12 months.

Stability Test: The thermal stability is described by the loss rate of thetargetprotein. The loss rate was determined by accelerated thermal degradationtest,that is, incubate the protein at 37°C for 48h, and no obvious degradationandprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this proteinislessthan 5% within the expiration date under appropriate storage condition.

[ SEQUENCES ]

The target protein is fused with two N-terminal Tags, His-tag and T7-tag, itssequence is listed below.

MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF- PEAVDFL ECANEARKKINSWVKTQTKG EIPNLLPEGS VDEDTKMVLV NTIYFKGRWK TPFQKRLNGL YPFRVNLNESKPVQMMYLRE KLNIGYIKDL KTQILELPYI GNISMFLLLP DEIEDSSTGL EMLEREINFDNFNKWISKET LDEDDVLVYI PKFKLAQNYE LKPILQRMGM EDAFNKGKAD FSGMSESNDLFLSEVFHQAT VDVNEEGTVA AGGTGAVMTG RTGHGGPQFV ADHPFLFFIMNNITRTIL

[ IDENTIFICATION ]

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