Numb同源物(NUMB)重组蛋白(Mouse,小鼠) 

Recombinant Numb Homolog (NUMB)

Protein S171

[ PROPERTIES ]

Host: E.coli

Residues: Leu377~Ser642 , with two N-terminal Tags, His-tag and T7-tag.

Subcellular Location: Membrane; Peripheral membrane protein.

Purity: >95% 

Endotoxin Level: <1.0EU per 1μg (determined by the LAL method).

Formulation: Supplied as lyophilized form in PBS, pH7.4, containing 5% sucrose, 0.01% sarcosyl.

Applications: SDS-PAGE; WB; ELISA; IP.

(May be suitable for use in other assays to be determined by the end user.)

Predicted isoelectric point: 8.1

Predicted Molecular Mass: 31.6kDa

[ USAGE ]

Reconstitute in sterile PBS, pH7.2-pH7.4.

[ STORAGE AND STABILITY ]

Storage:Avoid repeated freeze/thaw cycles.

Store at 2-8°C for one month.

Aliquot and store at -80°C for 12 months.

Stability Test: The thermal stability is described by the loss rate of thetarget protein. The loss rate was determined by accelerated thermal degradationtest, that is, incubate the protein at 37oC for 48h, and no obvious degradationandprecipitation were observed. (Referring from China Biological Products Standard, which was calculated by the Arrhenius equation.) The loss of this proteinislessthan 5% within the expiration date under appropriate storage condition.

[ SEQUENCES ]

The target protein is fused with two N-terminal Tags, His-tag and T7-tag, itssequence is listed below.

MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF - LTAK PANTALAHVAMP V RE T NPWA HV P D A A NK EI AAI HP G TEW G QS S GA A S P G L FQA G H R RTP SEADRWLEEVS KSVRAQQPQV SAAPLQPVLQ PPPPAAIAPP APPFQGHAFL TSQPVPVGVVPPLQPAFVPT QSYPVANGMP YPASNVPVVG ITPSQMVANV FGTAGHPQTT HPHQSPSLAKQQTFPQYETS SATTSPFFKP PAQHLNGSAA FNGVDNGGLA SGNRHAEVPP GTCPVDPFEAQWAALESKSK QRTNPSPTNP FS

[ IDENTIFICATION ]

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