颗粒酶M(GZMM)重组蛋白 (Rat,大鼠) 

Recombinant Granzyme M (GZMM)

GZM-M; LMET1; MET1; Hu-Met-1; Met-ase; Lymphocyte Met Ase 1; Met-1 serine protease; Natural killer cell granular protease

[ PROPERTIES ]

Host: E.coli

Residues: Ala19~Arg251 ,with two N-terminal Tags, His-tag and GST-tag.

Subcellular Location: Secreted. Cytoplasmicgranule.

Purity: >95%

Endotoxin Level: <1.0EU per 1μg(determined by the LAL method).

Formulation: Supplied as lyophilized form in PBS,pH7.4, containing 5% sucrose, 0.01% sarcosyl.

Applications: SDS-PAGE; WB; ELISA; IP.

(May be suitable for use in other assays to be determined by the end user.)

Predicted isoelectric point: 8.9

Predicted Molecular Mass: 57.4kDa

[ USAGE ]

Reconstitute in sterile PBS, pH7.2-pH7.4.

[ STORAGE AND STABILITY ]

Storage:Avoid repeated freeze/thaw cycles.

Store at 2-8°C for one month.

Aliquot and store at -80°C for 12 months.

Stability Test: The thermal stability is described by the loss rate of thetargetprotein. The loss rate was determined by accelerated thermal degradationtest,that is, incubate the protein at 37°C for 48h, and no obvious degradationandprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this proteinislessthan 5% within the expiration date under appropriate storage condition.

[ SEQUENCES ]

The target protein is fused with two N-terminal Tags, His-tag and GST-tag, itssequence is listed below.

MSPILGYWKI KGLVQPTRLL LEYLEEKYEE HLYERDEGDK WRNKKFELGL EFPNLPYYIDGDVKLTQSMA IIRYIADKHN MLGGCPKERA EISMLEGAVL DIRYGVSRIA YSKDFETLKVDFLSKLPEML KMFEDRLCHK TYLNGDHVTH PDFMLYDALD VVLYMDPMCL DAFPKLVCFKKRIEAIPQID KYLKSSKYIA WPLQGWQATF GGGDHPPKSD GSTSGSGHHH HHHSAGLVPRGSTAIGMKET AAAKFERQHM DSP DLGTLEV LFQGP LGSEF -AQIIGGREAVP HSRPYMVSLQN TKSHMCGGVL VHQKWVLTAA HCLSEPLQQL KLVFGLHSLH DPQDPGLTFYIKQAIKHPGY NLKYENDLAL LKLDGRVKPS KNVKPLALPR KPRDKPAEGS RCSTAGWGITHQ R G Q LA K S L QE L D L R L L D T RM C N NS RFW N GV LT DSM L C L KA GA KG QA P CKGDSGGPLVC GKGKVDGILS FSSKNCTDIF KPTVATAVAP YSSWIRKVIG R

[ IDENTIFICATION ]

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