神经纤维网蛋白2(NRP2)重组蛋白(Rat,大鼠) 

Recombinant Neuropilin 2 (NRP2)

NP2; NPN2; VEGF165R2; VEGF165-R2; Vascular endothelial cell growth factor 165 receptor 2

[ PROPERTIES ]

Source: Prokaryotic expression

Host: E.coli

Residues: Glu652~Pro858

Tags: Two N-terminal Tags, His-tag and GST-tag

Tissue Specificity: Brain, Liver.

Subcellular Location: Membrane; Single-pass type I membrane protein.

Purity: >98% 

Traits: Freeze-dried powder

Buffer formulation: 20mM Tris, 150mM NaCl, pH8.0, containing 1mMEDTA, 1mM DTT, 0.01% sarcosyl, 5%Trehalose and Proclin300.

Original Concentration: 200μg/mL

Applications: Positive Control; Immunogen; SDS-PAGE; WB.

(May be suitable for use in other assays to be determined by the end user.)

Predicted isoelectric point: 5.3

Predicted Molecular Mass: 53.3kDa

Accurate Molecular Mass: 58kDa as determined by SDS-PAGE reducingconditions.

Phenomenon explanation: 

The possible reasons that the actual band size differs from the predicted are as follows:

1.Splice variants: Alternative splicing may create different sized proteins from the same gene.

2. Relative charge: The composition of amino acids may affects the charge of the protein.

3. Post-translational modification: Phosphorylation, glycosylation, methylation etc.

4. Post-translation cleavage: Many proteins are synthesized as pro-proteins, and then cleavedto give the active form.

5. Polymerization of the target protein: Dimerization, multimerization etc.

[ USAGE ]

Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0mg/mL. Do not vortex.

[ STORAGE AND STABILITY ]

Storage:Avoid repeated freeze/thaw cycles.

Store at 2-8°C for one month.

Aliquot and store at -80°C for 12 months.

Stability Test: The thermal stability is described by the loss rate. The loss rate was determinedby accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and noobvious degradation and precipitation were observed. The loss rate is less than 5% within theexpiration date under appropriate storage condition.

[ SEQUENCE ]

[ IDENTIFICATION ]

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