瘦素(LEP)重组蛋白(Rat,大鼠)

Recombinant Leptin (LEP)

OB; OBS; Obesity Homolog; Obesity Factor; Obese Protein

[ PROPERTIES ]

Host: E.coli

Residues: Val22~Cys167 ,with two N-terminal Tags, His-tag and T7-tag.

Subcellular Location: Secreted

Purity: >95% 

Endotoxin Level: <1.0EU per 1μg(determined by the LAL method)

formulation: Supplied as lyophilized form in PBS,pH7.4, containing 5% trehalose, 0.01% sarcosyl.

Predicted isoelectric point: 6.6

Predicted Molecular Mass: 20.0kDa

Applications: SDS-PAGE; WB; ELISA; IP.(May be suitable for use in other assays to be determined by the end user.)

[ USAGE ]

Reconstitute in sterile PBS, pH7.2-pH7.4.

[ STORAGE AND STABILITY ]

Storage:Avoid repeated freeze/thaw cycles.

Store at 2-8°C for one month.

Aliquot and store at -80°C for 12 months.

Stability Test: The thermal stability is described by the loss rate of thetargetprotein. The loss rate was determined by accelerated thermal degradationtest,that is, incubate the protein at 37°C for 48h, and no obvious degradationandprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this proteinislessthan 5% within the expiration date under appropriate storage condition

[ SEQUENCES ]

The target protein is fused with two N-terminal Tags, His-tag and T7-tag, itssequence is listed below.MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF-VPIHKVQDDTKTLIKTIVTRINDISHTQS VSARQRVTGL DFIPGLHPIL SLSKMDQTLA VYQQILTSLP SQNVLQIAHDLENLRDLLHL LAFSKSCSLP QTRGLQKPES LDGVLEASLY STEVVALSRL QGSLQDILQQLDLSPEC

[ IDENTIFICATION ]

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