防御素α1B(DEFa1B)重组蛋白 (Human,人) 

Recombinant Defensin Alpha 1B (DEFa1B)

HNP-1; DEFA2; MRS; Neutrophil defensin 1

[ PROPERTIES ]

Source: Prokaryotic expression. 

Host: E. coli

Residues: Glu20~Cys94

Tags: Two N-terminal Tags, His-tag and GST-tag

Tissue Specificity: Kidney, Spleen. 

Subcellular Location: Secreted. 

Purity: >90%

Traits: Freeze-dried powder

Buffer formulation: 20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% sarcosyl, 5%Trehalose and Proclin300. 

Original Concentration: 200μg/mL

Applications: Positive Control; Immunogen; SDS-PAGE; WB. 

(May be suitable for use in other assays to be determined by the end user.)

Predicted isoelectric point: 5.7

Predicted Molecular Mass: 38.2kDa

Accurate Molecular Mass: 42kDa as determined by SDS-PAGE reducing conditions. 

Phenomenon explanation:

The possible reasons that the actual band size differs from the predicted are as follows:

1. Splice variants: Alternative splicing may create different sized proteins from the same gene. 

2. Relative charge: The composition of amino acids may affects the charge of the protein. 

3. Post-translational modification: Phosphorylation, glycosylation, methylation etc. 

4. Post-translation cleavage: Many proteins are synthesized as pro-proteins, and then cleaved to give the active form. 

5. Polymerization of the target protein: Dimerization, multimerization etc. 

[ USAGE ]

Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0mg/mL. Do not vortex.

[ STORAGE AND STABILITY ]

Storage: Avoid repeated freeze/thaw cycles.

Store at 2-8℃ for one month.

Aliquot and store at -80℃ for 12 months.

Stability Test: The thermal stability is described by the loss rate. The loss rate was determinedby accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and noobvious degradation and precipitation were observed. The loss rate is less than 5% within theexpiration date under appropriate storage condition.

[ SEQUENCE ]

[ IDENTIFICATION ]

Figure 1. SDS-PAGE

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