胎盘生长因子(PLGF)重组蛋白(Rat,大鼠) 

Recombinant Placenta Growth Factor (PLGF)

PlGF2; PGF; PGFL; Placental Growth Factor-Like; Vascular Endothelial Growth Factor-Related Protein

[ PROPERTIES ]

Host: E.coli

Residues: Ala24~Leu158 ,with two N-terminal Tags, His-tag and T7-tag.

Subcellular Location: Secreted

Purity: >95%

Endotoxin Level: <1.0EU per 1μg(determined by the LAL method).

Formulation: Supplied as lyophilized form in PBS,pH7.4, containing 5% sucrose, 0.01% sarcosyl.

Applications: SDS-PAGE; WB; ELISA; IP.

(May be suitable for use in other assays to be determined by the end user.)

Predicted isoelectric point: 7.7

Predicted Molecular Mass: 19.0kDa

[ USAGE ]

Reconstitute in sterile PBS, pH7.2-pH7.4.

[ STORAGE AND STABILITY ]

Storage:Avoid repeated freeze/thaw cycles.

Store at 2-8°C for one month.

Aliquot and store at -80°C for 12 months.

Stability Test: The thermal stability is described by the loss rate of thetargetprotein. The loss rate was determined by accelerated thermal degradationtest,that is, incubate the protein at 37°C for 48h, and no obvious degradationandprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this proteinislessthan 5% within the expiration date under appropriate storage condition.

[ SEQUENCES ]

The target protein is fused with two N-terminal Tags, His-tag and T7-tag, itssequence is listed below.

MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF- ALSAGNN STEMEVVPFNEVWGRSYCRP MEKLVYIADE HPNEVSHIFS PSCVLLSRCS GCCGDEGLHCVALKTANITMQILKIPPNRD PHSYVEMTFS QDVLCECRPI LETTKAERRK TKGKRKQSKT PQTEEPHL

[ REFERENCES ]

1. Dunne J.V., et al. (2013) Rheumatol. Int. 33:475-484.

2. Maae E., et al. (2012) Breast Cancer Res. Treat. 133:257-265.

3. Bobic S., et al. (2012) Am. J. Respir. Cell Mol. Biol. 46:781-789.

4. Xu Y., et al. (2012) Neurochem. Res. 37:665-670.

[ IDENTIFICATION ]

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