磷脂酰肌醇转运蛋白β(PITPNb)重组蛋白(Human,人) 

Recombinant Phosphatidylinositol Transfer Protein Beta (PITPNb)

PITPN-B; PI-TP-beta; PtdInsTP; VIB1B; PtdIns transfer protein beta

[ PROPERTIES ]

Source: Prokaryotic expression. 

Host: E. coli

Residues: Val2~Val271

Tags: N-terminal His-Tag

Tissue Specificity: Brain. 

Subcellular Location: Cytoplasm. Golgi apparatus. 

Purity: >94%

Traits: Freeze-dried powder

Buffer formulation: 100mM NaHCO₃, 500mM NaCl, pH8.3, containing 1mM EDTA, 1mM DTT, 0.01% sarcosyl and 5%Trehalose. 

Original Concentration: 200μg/mL

Applications: Positive Control; Immunogen; SDS-PAGE; WB. 

(May be suitable for use in other assays to be determined by the end user.)

Predicted isoelectric point: 6.4

Predicted Molecular Mass: 35.1kDa

Accurate Molecular Mass: 39kDa as determined by SDS-PAGE reducing conditions. 

Phenomenon explanation:

The possible reasons that the actual band size differs from the predicted are as follows:

1.Splice variants: Alternative splicing may create different sized proteins from the same gene.

2. Relative charge: The composition of amino acids may affects the charge of the protein.

3. Post-translational modification: Phosphorylation, glycosylation, methylation etc.

4. Post-translation cleavage: Many proteins are synthesized as pro-proteins, and then cleaved to give the active form.

5. Polymerization of the target protein: Dimerization, multimerization etc.

[ USAGE ]

Reconstitute in 100mM NaHCO₃, 500mM NaCl (pH8.3) to a concentrationof0.1-1.0 mg/mL. Do not vortex.

[ STORAGE AND STABILITY ]

Storage: Avoid repeated freeze/thaw cycles.

Store at 2-8℃ for one month.

Aliquot and store at -80℃ for 12 months.

Stability Test: The thermal stability is described by the loss rate. The loss rate was determinedby accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and noobvious degradation and precipitation were observed. The loss rate is less than 5% within theexpiration date under appropriate storage condition.

[ SEQUENCE ]

[ IDENTIFICATION ]

Figure 1. SDS-PAGE

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