胰岛素样生长因子结合蛋白1(IGFBP1)重组蛋白 (Pig,猪) 

Recombinant Insulin Like Growth Factor Binding Protein 1 (IGFBP1)

IBP1; AFBP; IGF-BP25; PP12; Placental Protein 12; Amniotic Fluid Binding Protein; Alpha-Pregnancy-Associated Endometrial Globulin; Growth Hormone Independent-Binding Protein

[ PROPERTIES ]

Host: E.coli

Residues: : Glu112~Asn262 ,with two N-terminal Tags, His-tag and T7-tag.

Subcellular Location: Secreted

Purity: >95%

Endotoxin Level: <1.0EU per 1μg(determined by the LAL method).

Formulation: Supplied as lyophilized form in PBS,pH7.4, containing 5% sucrose, 0.01% sarcosyl.

Applications: SDS-PAGE; WB; ELISA; IP.

(May be suitable for use in other assays to be determined by the end user.)

Predicted isoelectric point: 6.4

Predicted Molecular Mass: 21.2kDa

[ USAGE ]

Reconstitute in sterile PBS, pH7.2-pH7.4.

[ STORAGE AND STABILITY ]

Storage:Avoid repeated freeze/thaw cycles.

Store at 2-8°C for one month.

Aliquot and store at -80°C for 12 months.

Stability Test: The thermal stability is described by the loss rate of thetargetprotein. The loss rate was determined by accelerated thermal degradationtest,that is, incubate the protein at 37°C for 48h, and no obvious degradationandprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this proteinislessthan 5% within the expiration date under appropriate storage condition.

[ SEQUENCES ]

The target protein is fused with two N-terminal Tags, His-tag and T7-tag, itssequence is listed below.

MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF-EATETKDPA APETTSPESTEMTQEQLLDS FHLMATSSED LPILWNAINN YESMKALEAT DIKKWKEPCQRELYKVLDRLAREQQKAGD R LYKFY LP NCN KNGFY HS KQC ETS LEGEPG L CW CV YPWSGKKILGSTAVRG DPKCHQYFNS QN

[ IDENTIFICATION ]

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