着丝粒蛋白J(CENPJ)重组蛋白(Mouse,小鼠) 

Recombinant Centromere Protein J (CENPJ)

LAP; LIP1; BM032; CPAP; MCPH6; Microcephaly,Primary Autosomal Recessive 6; Centrosomal P4.1-associated protein; LAG-3-associated protein; LYST-interacting protein 1

[ PROPERTIES ]

Source: Prokaryotic expression

Host: E.coli

Residues: Met1~Asp204

Tags: N-terminal His Tag

Subcellular Location: Cytoplasm

Purity: > 95% 

Traits: Freeze-dried powder

Buffer formulation: 100mMNaHCO₃, 500mMNaCl, pH8.3, containing 0.01% SKL, 5%Trehalose.

Original Concentration: 200μg/mL

Applications: Positive Control; Immunogen; SDS-PAGE; WB.

(May be suitable for use in other assays to be determined by the end user.)

Predicted isoelectric point: 5.2

Predicted Molecular Mass: 26.8kDa

Accurate Molecular Mass: 30kDa as determined by SDS-PAGE reducing conditions.

Phenomenon explanation: 

The possible reasons that the actual band size differs from the predicted are as follows:

1.Splice variants: Alternative splicing may create different sized proteins from the same gene.

2. Relative charge: The composition of amino acids may affects the charge of the protein.

3. Post-translational modification: Phosphorylation, glycosylation, methylation etc.

4. Post-translation cleavage: Many proteins are synthesized as pro-proteins, and then cleavedto give the active form.

5. Polymerization of the target protein: Dimerization, multimerization etc.

[ USAGE ]

Reconstitute in 100mM NaHCO₃, 500mM NaCl (pH8.3) to a concentration of 0.1-1.0 mg/mL. Donot vortex

[ STORAGE AND STABILITY ]

Storage:Avoid repeated freeze/thaw cycles.

Store at 2-8°C for one month.

Aliquot and store at -80°C for 12 months.

Stability Test: The thermal stability is described by the loss rate. The loss rate was determinedby accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and noobvious degradation and precipitation were observed. The loss rate is less than 5% within theexpiration date under appropriate storage condition.

[ SEQUENCE ]

[ IDENTIFICATION ]

[ IMPORTANT NOTE ]

The kit is designed for research use only, we will not be responsible for any issue if the kit wasused in clinical diagnostic or any other procedures.

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